An immunoblot technique for the detection of lymphocystis disease virus (LCDV) in naturally infected gilt-head seabream (Sparus aurata, L.) has been developed. A specific antiserum against a 60 kDa viral protein has been proven to be an appropriate tool for LCDV diagnosis either from inoculated cell cultures or from fish tissues using the immunoblot assay. The sensitivity of this technique vari...

The polypeptide and glycoprotein composition of eight strains of the fish-pathogenic lymphocystis disease virus (LCDV) isolated from gilt-head seabream (Sparus aurata), blackspot seabream (Pagellus bogaraveo), and sole (Solea senegalensis) were determined. The protein electrophoretic patterns of all LCDV isolates were quite similar regardless of the host fish, showing two major proteins (79.9 a...

The effects of the dietary administration of two heat-inactivated whole bacteria from the Vibrionaceae family, singly or combined, on innate immune response of the seabream were studied. The two bacteria (Pdp11 and 51M6), which were obtained from the skin of gilthead seabream, showed in vitro characteristics that suggested they could be considered as potential fish probiotics. The fish were fed...

Feeding cycles entrain biological rhythms, which enabling animals to anticipate feeding times and so maximize food utilization. In this article the effect of mealtime on locomotor activity, blood glucose, gastric pH and digestive enzymes was studied in two groups of seabream (Sparus aurata): one group received a single daily meal at random times either during the light and the dark period (rand...

Life cycle assessment is used in this study to evaluate the potential environmental impacts of producing gilthead seabream in an offshore sea farm exposed to storms in the Spanish Mediterranean Sea. The farming methods used can be considered as “basic” since no centralized or automatic food distribution system exists and there is no control system. This study aims to identify the main hotspots ...

Knowing that food ingestion and digestion are processes under neuroendocrine regulation, it is important to understand how fish larvae regulate these processes, when the digestive system itself development. This study aimed analyze gilthead seabream (Sparus aurata) larval feeding incidence intensity were affected by time, light, water temperature, through analysis of gut content this cholecysto...

In this study the influence of the dietary level of the fatty acid arachidonic acid (ArA, 20:4n-6) was determined on the acute stress response and osmoregulation of adult gilthead seabream Sparus aurata L. Seabream were fed a diet containing either 0.9% or 2.4% of total fatty acids as ArA for 18 days before being subjected to a 5 min period of net confinement. Prior to this stressor, a subgroup...

The present research looked at the ability of gilthead seabream to discriminate between two similar diets differing only in the extent of lipid oxidation. Six groups of 4 fish (254g initial wet weight) were allowed to select by means of self-feeders between two complete diets (34.5% casein + gelatin, 14.8% fish oil + soybean oil, 24.8% dextrin and 25.9% vitamins, minerals, filler and binder) wh...

The skin is the first barrier of defence in fish, protecting against any external stressor and preserving the integrity and homeostasis of the fish body. The aim of this study was to characterise gilthead seabream skin by isolating cells and studying the cell cycle by flow cytometry, to study the skin histology by scanning electron microscopy and the transcription level of some immune-relevant ...

Ž . The performance of microdiets MDs for larvae of marine fish is frequently improved when they are co-fed with Artemia. This suggests that nutritional factors in the live food are positively influencing the ingestion, digestion and assimilation of the MD. This paper reviews recent advances in MD development on the gilthead seabream with special emphasis on studies that isolated, identified an...