Bacterial luciferase and NADH:FMN oxidoreductase have been immobilized onto arylamine glass beads. These immobilized enzymes can detect as little as 0.2 pmol of NADH per assay sample. Glucose-6-phosphate dehydrogenase has been co-immobilized with these enzymes, and with this system it is possible to quantitate 1 pmol of glucose 6-phosphate. By co-immobilizing a fourth enzyme, hexokinase, onto t...

CO2 hydrate formation and dissociation is crucial for hydrate-based CO2 capture and storage. Experimental and calculated phase equilibrium conditions of carbon dioxide (CO2) hydrate in porous medium were investigated in this study. Glass beads were used to form the porous medium. The experimental data were generated using a graphical method. The results indicated the decrease of pore size resul...

While granular segregation in partially filled containers has been studied extensively, dynamics densely spheres is not fully understood. Here, surface band and convection are reported a rotating sphere of highly compacted glass beads. Distinct from the Rayleigh–Bénard convection, butterfly shaped structure with vortexes alternating layers small/large beads, which stable independent size. Two c...

This study examines differences between chemisorbed and physisorbed biomolecules on bacterial adhesion to both hydrophobic and hydrophilic surfaces that are biologically nonspecific. Bacteria-sized latex microspheres were used as a simplified model in order to study these factors that affect microbial adhesion. Two biomolecules (protein A, poly-D-lysine) were covalently bound to microspheres in...

The hydrodynamic behaviour of air-glass beads bubbling fluidized bed reactor containing spherical glass is numerically studied, using OpenFoam v7 CFD software. Both Gidaspow and Syamlal-O'Brien drag models are used to calculate momentum exchange coefficients. Simulation predictions pressure loss, expansion rate, air volume fraction parameters were compared validated data, existing in the litera...

Bacterial surfaces contain proteins, polysaccharides, and other biopolymers that can affect their adhesion to another surface. To better understand the role of proteins in bacterial adhesion, the interactions between two different model colloids (glass beads and carboxylated latex microspheres) and four proteins covalently bonded to glass surfaces were examined using colloid probes and an atomi...

Using an experimental setup based on packed bed reactors (filled with glass beads) supplied with water from a groundwater source, the suitability of different microscopic techniques for biofilm monitoring was evaluated. Confocal microscopy is the most versatile, applicable and suitable technology for biofilm study and even provides information about the main surface and height parameters. Never...

To show the effects of the particles and forced disturbances on the instantaneous large-scale vortex structures in a gas-particle round jet, coherent structures in gas-particle turbulent round jets were investigated experimentally by flow visualization. The 45m and 350m diameter glass beads were used as the particles in the experiments. An acoustic speaker was used to introduce velocity perturb...

We present an experimental and theoretical investigation of the Generalized Einstein Relation (GER), a particular form of a fluctuation-dissipation relation, in an out-of-equilibrium visco-elastic fluid. Micrometer beads, used as thermometers, are immersed in an aging colloidal glass to provide both fluctuation and dissipation measurements. The deviations from the Generalized Einstein Relation ...