We have studied the effects of first and second generation sulphonylureas on the release of insulin and neuropeptide tyrosine (NPY) from hamster insulinoma tumour (HIT T15) cells and isolated rat islets. In the presence of 5.5 mmol/l glucose all sulphonylureas stimulated insulin release from the HIT cells (P<0.01 ANOVA, n> or =4) but only glibenclamide (GLIB, 10 micromol/l) stimulated the relea...

An environmentally benign RP-HPLC approach for rapid analysis of glibenclamide in pure form, developed nanoemulsion and commercial tablets was developed and validated in present investigation. The green chromatographic identification was performed on Lichrosphere 250 X 4.0 mm RP C8 column having a 5 μm packing as a stationary phase using a combination of ethanol: methanol (50:50 % v/v) as a mob...

Intrahepatic cholestasis and cutaneous bullae associated with glibenclamide therapy are described in a 61-year-old diabetic patient who presented wit hypoglycaemic coma. These features have not previously been reported as side effects of glibenclamide therapy, but intrahepatic cholestasis may occur with chlorpropamide, a similar sulphonylurea agent. The mechanism of this cholestasis is not clea...

We have demonstrated that myocardial ATP-sensitive potassium (K(ATP)) channels are implicated in the development of cardiac hypertrophy in hyperlipidemic rabbits. We investigated the effect of pravastatin on development of ventricular hypertrophy in male normolipidemic Wistar rats with two-kidney, one-clip (2K1C) hypertension and whether the attenuated hypertrophic effect was via activation of ...

Matar, W., T. M. Nosek, D. Wong, and J.-M. Renaud. Pinacidil suppresses contractility and preserves energy but glibenclamide has no effect during muscle fatigue. Am. J. Physiol. Cell Physiol. 278: C404–C416, 2000.—The effects of 10 μM glibenclamide, an ATP-sensitive K1 (KATP) channel blocker, and 100 μM pinacidil, a channel opener, were studied to determine how the KATP channel affects mouse ex...

Van Lunteren, Erik, Michelle Moyer, and Augusto Torres. ATP-sensitive K1 channel blocker glibenclamide and diaphragm fatigue during normoxia and hypoxia. J. Appl. Physiol. 85(2): 601–608, 1998.—The role of ATP-sensitive K1 channels in skeletal muscle contractile performance is controversial: blockers of these channels have been found to not alter, accelerate, or attenuate fatigue. The present s...

BACKGROUND Sepsis is a common disease that continues to increase in incidence in the world. Diseases, such as diabetes mellitus, may make the situation worse. Diabetic patients are at increased risk for common infections. This study was designed to investigate the role of glibenclamide on myocardial injury by lipopolysaccharides (LPS) in streptozotocin induced diabetic mice (STZ-mice). METHOD...

OBJECTIVE This study evaluated the anti-hyperglycemic effect of the methanol extract of Gongronema latifolium leaves singly and in combination with an oral hypoglycemic agent; glibenclamide. MATERIALS AND METHODS The plant material was extracted with methanol for 48 h using cold maceration and concentrated in vacuo in a rotary evaporator. The methanol extract of G. latifolium at doses of 200,...

Recent work has established membrane phospholipids such as phosphatidylinositol-4,5-bisphosphate (PIP(2)) as potent regulators of K(ATP) channels controlling open probability and ATP sensitivity. We here investigated the effects of phospholipids on the pharmacological properties of cardiac type K(ATP) (Kir6.2/SUR2A) channels. In excised membrane patches K(ATP) channels showed considerable varia...