Evidence is presented that the resistance of Aspergillus nidulans hyphae to lysis by a beta-(1-->3) glucanase-chitinase mixture results from the presence of melanin in the fungal walls. The resistance of the walls to digestion was directly correlated with the melanin content of the mycelium. A melanin-less mutant of A. nidulans was highly susceptible to hydrolysis by the enzyme mixture. Preincu...

An endo-a-D-(1 + 3).glucanase capable of hydrolyzing various ~(1 -+ 3).glucans has been isolated and purified from the culture filtrate of the cellulolytic fungus Trichoderma viride, and its specificity has been examined. Of the compounds tested only those with oc-(1 ---f 3).glucosidic linkages were attacked, and the enzymatic hydrolysis occurred with retention of configuration of the anomeric ...

The low nutritional value of barley for poultry is because of the absence of an intestinal enzyme for efficient depolymerization of (1, 3-1,4)-beta-glucan, the major polysaccharide of the endosperm cell walls. This leads to high viscosity in the intestine, limited nutrient uptake, decreased growth rate, and unhygienic sticky droppings adhering to chickens and floors of the production cages. Con...

The objective was to investigate the effect of supplementing a wheat distillers dried grains with solubles (wheat DDGS)-based diet with carbohydrase enzyme blends on growth performance and nutrient digestibilities in growing and finishing pigs. In Exp. 1, 48 pigs with a BW of 36.5 +/- 1.03 kg (mean +/- SD) were randomly assigned to 24 pens (2 pigs per pen) on the basis of sex and BW, and pigs w...

Three-week-old broiler chicks and 1-yr-old Leghorn cocks were used to determine the effect of beta-glucanase addition (experimental product from Trichoderma longibrachiatum) to a 60% barley-based diet on rate of feed passage, transit time, and mean retention time. Fecal chromium oxide content was used to calculate cumulated and noncumulated excretion data. A Hill equation was chosen to test the...

the present study was carried out to investigate the effect of different levels of triticale with/ without enzyme cocktail (xylanase and β-glucanase) on performance of broiler chicks. five hundred day old male broiler chicks (ross 308), assigned to a factorial arrangement (5×2) with a completely randomized design with 5 replicates of 10 chicks each. the factors included 5 levels of triticale (0...

The endo-β-1,4-glucanase gene celE from the anaerobic fungus Orpinomyces PC-2 was placed under the control of an alcohol oxidase promoter (AOX1) in the plasmid pPIC9K, and integrated into the genome of a methylotrophic yeast P. pastoris GS115 by electroporation. The strain with highest endo-β-1,4-glucanase activity was selected and designed as P. pastoris egE, and cultivated in shaking flasks. ...

This study was performed with broiler chickens to evaluate the effects of adding beta-glucanase on bird performances and digestive organs weights. A total of 1600 1-day-old broilers (Arbor Acres) were divided into 2 groups and fed either a control diet (group C) based on corn, wheat and barley or a control diet supplemented with beta-glucanase preparation (Safizym GP 40, Lesaffre Feed Additives...