Sterylglucosides (SGs) are composed of a glucose and sterol derivatives, and are distributed in fungi, plants and mammals. We recently identified EGCrP1 and EGCrP2 (endoglycoceramidase-related proteins 1 and 2) as a β-glucocerebrosidase and steryl-β-glucosidase, respectively, in Cryptococcus neoformans. We herein describe an EGCrP2 homologue (Egh1; ORF name, Yir007w) involved in SG catabolism i...

This study aimed to investigate the potential use of anthocyanin Berberis crataegina DC. as a natural food coloring agent in industry. For this aim, ultrasound-assisted extraction (UAE) method was performed extract The effect ultrasound power (X1: 20-100%), temperature (X2: 20-60 °C), and time (X3: 10-20 min) on TPC TAC extracts were examined optimized by applying Box–Behnken experimental desig...

The discovery of an acylated steryl glucoside in plant tissue in 1964 (13) raised the number of known forms of sterols in plants to four. These are the free sterols, sterol esters, steryl glycosides, and acylated steryl glycosides. Kiribuchi et al. (12) analyzed the sterol content of soybean and found that sterol ester was present in very small amount and that the sterol was mostly in the form ...

Although cytokinins (CKs) affect a number of processes connected with chloroplasts, it has never been rigorously proven that chloroplasts contain CKs. We isolated intact chloroplasts from tobacco (Nicotiana tabacum L. cv SR1) and wheat (Triticum aestivum L. cv Ritmo) leaves and determined their CKs by liquid chromatography/tandem mass spectroscopy. Chloroplasts from both species contained a who...

Recent experimental data point to an interaction of dietary flavonol monoglucosides with the intestinal Na-dependent glucose transporter 1 (SGLT1). To investigate this interaction in more detail, we performed experiments with SGLT1-containing brush-border-membrane vesicles (BBMV) from pig jejunum. The flavonol quercetin-3-O-glucoside (Q3G) concentration-dependently inhibited Na-dependent uptake...

BACKGROUND Sugarcane molasses is a potential by-product of the sugarcane manufacturing industry that is rich in antioxidant materials. The present study aimed to obtain antioxidative compounds from sugarcane molasses and to evaluate their ability to protect DNA from oxidative damage. RESULTS Two neolignan glucosides were isolated from sugarcane molasses using bioassay and UV spectra monitorin...

The aim of this study was to determine anthocyanin content and antioxidant activity of 20 different grape varieties, using HPLC and DPPH, FRAP and ABTS assays, respectively. The identified anthocyanins were malvidin-3-glucoside, delphinidin-3-glucoside, petunidin-3-glucoside, cyanidin3 glucoside, and peonidin-3-glucoside on the basis of their retention times. Comparatively the total anthocyanin...

Curcumin was converted into its oligosaccharides by cultured Parthenocissus tricuspidata cells-catalyzed glycosylation. In addition to curcumin 4'-O-glucoside (9%) and curcumin 4',4''-O-diglucoside (14%), two curcumin oligosaccharides, i.e., curcumin 4'-O-gentiobioside (16%) and curcumin 4'-O-gentiobiosyl-4''-O-glucoside (3%), were isolated from P. tricuspidata cells treated with curcumin. ____...

From the leaves of Alangium kurzii, a new tetrahydroisoquinoline-monoterpene glucoside, 6-O-methyl-N-deacetylipecosidic acid and a new iridoid glucoside, 10-O-benzoyladoxosidic acid, were isolated along with alangiside, demethylalangiside, 6''-O-beta-D-glucosylhenryoside, uridine and four known flavonoid glycosides. The structures of new glucosides were determined on the basis of spectroscopic ...

Two serotonin derivatives, N,N-dimethylserotonin 5-O-β-glucoside (1a) and N-methylserotonin 5-O-β-glucoside (1b) were isolated from immature seeds of Zanthoxylum piperitum. Their structures were determined by multi-step conversion reactions and spectroscopic analyses. Immature seeds of Z. piperitum contained extremely high levels of compounds 1a and 1b of approximately 0.29% and 0.15% (w/w), re...