A new micromethod for the determination of sphingomyelin in samples suspended in aqueous solutions, and modified micromethods for determining phosphatidylcholine and phosphatidylglycerol were used to determine phosphatidylcholine and sphingomyelin (detection limits of 1.8 mumol/l), and phosphatidylglycerol (detection limit of 2.3 mumol/l) in lipid dispersions, membranes from sheep erythrocytes ...

Using a new approach based on simultaneous measurements of net changes in glycerol and free fatty acids, the rates of lipolysis and of fatty acid esterification were estimated in rat epididymal fat pads under various conditions in vitro. Glycerol release, taken as a measure of the rate of lipolysis, was stimulated by epinephrine, norepinephrine, glucagon, adrenocorticotropic hormone (ACTH), thy...

against M. phlei and M. 8megmati8, using the medium of Dubos (1947), but the results were unsatisfactory, since the lower dilutions showed a turbidity which was undistinguishable from the growth of the organism. A point of minimum turbidity was noted however in each case, which may give some indication of the limiting dilution. This occurred with the triterpenoid acid at 1: 16,000 and with ungu...

A sulfur-containing glycolipid, accounting for ca. 25% of the total polar lipids, has been isolated from the extreme halophile Halobacterium cutirubrum. The ammonium salt of the lipid was found to have the molecular formula C(61)H(117)O(21)S.NH(4), and on strong acid hydrolysis it yielded 2,3-di-O-phytanyl-sn-glycerol, glucose, mannose, galactose, and sulfate in equimolar proportions. Infrared ...

The relative motion of two surfaces in direct contact results friction and wear. This affects every moving surface, contributing to a quarter the worldwide energy consumption. addition lubricant can reduce by separating surfaces, making more energy-efficient systems. Lubricants are composed base oil series additives. Molecules like glycerol improve efficiency system. However, hydrophilic molecu...

In vitro methods currently utilized for the determination of lipolytic responses in adipose tissue have not allowed for the continuous monitoring of rapid successive changes in rates of lipolysis. The perifused fat cell system utilized in this investigation offers a simple, reproducible method by which minute-to-minute changes in the rate of lipolysis of isolated fat cells may be monitored. In ...

Glycerol is a promising feedstock for microbial cultivation and production of 1,3 propanediol (1,3 PDO). Here we report a newly isolated bacterial strain BA11 from soil, capable of fermenting glycerol to 1,3 PDO, and has been identified to be a strain of Klebsiella pneumoniae. Strain BA11 was fast growing showing peak 1,3 PDO production in 6 h of cultivation with productivity of 1.2 g/...

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and have multiple effects in various tissues including adipose inflammation, a condition characterized by increased local release of the pro-lipolytic cytokine tumor necrosis factor-alpha (TNF-α). Whether miRNAs regulate adipocyte lipolysis is unknown. We set out to determine whether miRNAs affect adipocyte lipolysis in ...