Mannoproteins of approximately 50 kDa from blastoconidia and 60 kDa from hyphae of Candida albicans reacted in Western blots (immunoblots) with either a polyclonal rabbit antiserum (CA-7) or a monoclonal antibody (CA-A) to the C. albicans C3d-binding protein (complement receptor type 2). The glycosylated nature of these proteins was demonstrated by their reactivity with concanavalin A and by se...

The objective of this study was to evaluate the impact of hormonal status and bacterial vaginosis (BV) on the glycosidases present and glycosylation changes as assessed by lectin binding to cervicovaginal lavage constituents. Frozen cervicovaginal lavage samples from a completed study examining the impact of reproductive hormones on the physicochemical properties of vaginal fluid were utilized ...

Several glycosidases, purified and characterized from mammalian tissues, have been shown to be optimally active under acidic conditions when p-nitrophenyl (PNP) or 4-methylumbelliferyl glycosides are used as substrates. Although high levels of the glycosidases are present in the epididymal lumen, their physiological role remains uncertain. To be functional, the glycosidases are expected to be e...

Five of the six known glycosidases in the culture medium of Streptococcus pneumoniae have been purified from 600to 55,000-fold by a systematic procedure of ion exchange and affinity chromatography. Following partial separation of the glycosidases on DEAE-Sephadex, the neuraminidase, the endo-cu-N-acetylgalactosaminidase, the /3-galactosidase, and the P-iV-acetylglucosaminidase were further puri...

The reclusive enzymes that catalyze proteolysis inside the cell membrane are among the most widespread in nature, yet most remain ‘orphans’ whose cellular functions are poorly understood. Now, Voss et al identify Golgi-resident glycosyltransferases and glycosidases as substrates for the presenilin-like protease SPPL3. Shedding of these glycan-modifying enzymes from the membrane down-regulates g...