Genetically modified mice carrying engrafted human tissues provide useful models to study human cell biology in physiologically relevant contexts. However, there remain several obstacles limiting the compatibility of human cells within their mouse hosts. Among these is inadequate cross-reactvitiy between certain mouse cytokines and human cellular receptors, depriving the graft of important surv...

The implementation of flow cytometry assays in evaluation of transplant recipients began in 1983 when Garavoy et. al. applied this technology to study the in vitro production of HLA antibodies and observed that flow cytometric technology identified serum HLA antibody levels so low that the complement dependent cytotoxicity assays could not detect them. Since even low levels of antibodies have b...

The clinical relevance of humoral allosensitization has gained a lot of attention in the last few years. An increasing number of studies have demonstrated adverse graft survival in patients who have either preformed or post-transplant-developed anti-HLA antibodies. The detection of HLA antibodies and the specificity analysis have evolved over time from primarily cell-based to solid-phase method...

costochondral and rib grafts have long been used for reconstruction of lost condyle. donor site morbidity has less been under investigation and researched. pleural disruption pneumothorax and ugly scars are among the donor site complications. this study aims to determine the rate of pleural disruption following costochondral graft harvesting from the 4th to the 7th ribs of right and left ribs f...

Poly(L-lactic acid) (PLLA) chains were grafted on xyloglucan substrates via ring-opening polymerization of the L-lactide monomer. Different parameters such as the nature of the substrate (native or modified xyloglucan) and the substrate/monomer ratios were varied in the synthesis to achieve different lengths of the grafted chains. A range of experimental techniques including infrared spectrosco...

BACKGROUND In an attempt to prepare scaffolds with porosity and compressive strength as high as possible, we prepared porous β-tricalcium phosphate (TCP) scaffolds and coated them with regenerative medicine-grade gelatin. The effects of the gelatin coating on the compressive strength and in vivo osteoblast compatibility were investigated. METHODS Porous β-TCP scaffolds were prepared and coate...

THE occurrence of donor-specific cellmediated lympholysis (CML) nonreactivity in unrelated and related donor-recipient combinations has been documented in several reports." Our results showed that the development of CML nonreactivity correlated significantly with good graft function. Furthermore, we found that compatibility for the Η LA-Β locus antigens between donor and recipient and sex match...

INTRUCTION: Synthetic bone graft such as hydroxyapatite[(Ca10(PO4)6(OH)2] (HA) ceramic have been exhibited high tissue compatibility, osteoconductivity and resistance to infection. However, the insufficient bending strength of HA ceramic, probably too tight to bind of BMPs to HA limit its usage[1]. Various hydroxyapatite /collagen composites were developed as potential biomaterials for bone sub...

Methods to manipulate and visualize isolated DNA and oligonucleotide strands are important for investigation of their biophysics as well as their interactions with proteins. Herein, we report such a method by combining a block copolymer surface functionalization strategy with microfluidics. The copolymer poly(l-lysine-graft-polyethylene glycol) (PLL-g-PEG) coated one surface of the microfluidic...