Selection of cells positive for aldehyde dehydrogenase (ALDH) activity from a green-fluorescent background is difficult with existing reagents. Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for labelling viable ALDH(pos) cells. We demonstrate that AldeRed 588-A successfully isolates ALDH(hi) human haematopoietic stem cells from heterogeneous cord blood mononuclear ...

A fluorescent sensor that can monitor levels of extracellular ammonium has been made by using a fused green fluorescent protein to detect conformational changes in ammonium transport proteins.

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. ...

O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches. Here, we characterize several new BG fluorophores suitable for in vivo AGT labeling that display fluorescence emission maxima covering the visible spectrum from 472 to 673 nm, thereby extending th...

Bimolecular fluorescence complementation (BiFC) represents one of the most advanced and powerful tools for studying and visualizing protein-protein interactions in living cells. In this method, putative interacting protein partners are fused to complementary non-fluorescent fragments of an autofluorescent protein, such as the yellow spectral variant of the green fluorescent protein. Interaction...

Objective(s): Filamentous bacteriophage M13 was genetically engineered to specifically target mammalian cells for gene delivery purpose. Materials and Methods: A vascular endothelial growth factor receptor 2 (VEGFR2)-specific nanobody was genetically fused to the capsid gene III of M13 bacteriophage (pHEN4/3VGR19). A mammalian expression construct containing Cop-green fluorescent protein (Cop-G...

The advent of fluorescent proteins (FPs) has redefined the use of fluorescence microscopy in every biological discipline. Green fluorescent protein (GFP) and its variants are commonly used in celluar imaging studies. Recently, a novel fluorescent protein, named vivid Verde Fluorescent Protein (VFP), was isolated from Cyphastrea microphthalma, a scleractinian coral found in the warmer waters of ...

The advent of fluorescent proteins (FPs) has redefined the use of fluorescence microscopy in every biological discipline. Green fluorescent protein (GFP) and its variants are commonly used in celluar imaging studies. Recently, a novel fluorescent protein, named vivid Verde Fluorescent Protein (VFP), was isolated from Cyphastrea microphthalma, a scleractinian coral found in the warmer waters of ...

The advent of fluorescent proteins (FPs) has redefined the use of fluorescence microscopy in every biological discipline. Green fluorescent protein (GFP) and its variants are commonly used in celluar imaging studies. Recently, a novel fluorescent protein, named vivid Verde Fluorescent Protein (VFP), was isolated from Cyphastrea microphthalma, a scleractinian coral found in the warmer waters of ...

The advent of fluorescent proteins (FPs) has redefined the use of fluorescence microscopy in every biological discipline. Green fluorescent protein (GFP) and its variants are commonly used in celluar imaging studies. Recently, a novel fluorescent protein, named vivid Verde Fluorescent Protein (VFP), was isolated from Cyphastrea microphthalma, a scleractinian coral found in the warmer waters of ...