After sample processing for RNA and DNA analysis, the leftover protein pellets are usually discarded due to limited efficiency of pellet reconstitution/solubilisation. As pelleted proteins tightly packed, they most often solubilised using chaotropic agents (e.g., guanidine hydrochloride or urea), detergents SDS), salts (NaCl) basic buffer (Tris). The aim this study was define optimise procedure...

Background: The refolding of proteins from inclusion bodies is affected by several factors, including solubilization of inclusion bodies by denaturants, removal of the denaturant, and assistance of refolding by small molecule additives. Objectives: The purpose of this study was optimization of recombinant human interferon-b purification in order to achieve higher efficiency, yield, and a produ...

Bovine serum albumin, in aqueous guanidine hydrochloride, interacts preferentially with the solvent components, as was shown by techniques in which refractometry and light scattering and equilibrium dialysis were used. If constant salt molality after dialysis is taken as the reference state for zero binding, then 0.08 f 0.03 and 0.18 f 0.05 g of salt per g of protein is bound at 3 M and 6 M sal...

The denaturation and renaturation of bovine carbonic anhydrase B is a thermodynamically reversible process, uncomplicated by aggregation or disuhide bond formation. The reaction is less cooperative than is the unfolding and refolding of most globular proteins, in that distinct successive stages can be observed both in equilibrium and kinetic measurements. This enzyme is therefore ideally suited...

Homogeneous preparations of the potassium-requiring yeast aldehyde dehydrogenase are active with either DPN or TPN in the oxidation of a wide variety of aldehydes. Exogenous mercaptan is not a requirement for catalytic activity. The enzyme is denatured in 6 M guanidine hydrochloride to form a protein species with a molecular weight somewhat greater than 21,000. The denatured protein may be reas...

The majority of the phage-induced proteins made after T7 infection of Escherichia coli are tightly associated with the bacterial membrane. Many of these have been identified. Selective extraction of proteins from these membranes by the detergent Sarkosyl or the chaotropic agent guanidine-hydrochloride indicated that most of these proteins are an integral part of the cytoplasmic membrane and the...

The Bacillus thuringiensis subsp. israelensis mosquito larvicidal toxin is not a sulfhydryl-activated toxin. The protein disulfide bonds were cleaved and blocked without loss of toxicity. In contrast, modification of the lysine side chains eliminated toxicity. Additionally, the toxin was resistant to high concentrations of salt (8 M NaBr), organic solvents (40% methanol), denaturants (4 M urea)...