The liver, an organ with an exceptional regeneration capacity, carries out a wide range of functions, such as detoxification, metabolism and homeostasis. As such, hepatocytes are an important model for a large variety of research questions. In particular, the use of human hepatocytes is especially important in the fields of pharmacokinetics, toxicology, liver regeneration and translational rese...

In this paper we discuss the differences between the average marginal effect and the marginal effect of the average individual in sample selection models, estimated by Heckman’s two step procedure. We show that the bias that emerges as a consequence of interchanging them, could be very significant, even in the limit. We suggest a computationally cheap approximation method, which corrects the bi...

For situations where the number of tested hypotheses is increasingly large, the power to detect statistically significant multiple treatment effects decreases. As is the case with microarray technology, often researchers are interested in identifying differentially expressed genes for more than two types of cells or treatments. A two-step procedure is proposed for the purpose of increasing powe...

Actin-crosslinking proteins link F-actin into the bundles and networks that constitute the cytoskeleton. Dystrophin, beta-spectrin, alpha-actinin, ABP-120, ABP-280, and fimbrin share homologous actin-binding domains and comprise an actin crosslinker superfamily. We have identified a novel member of this superfamily (ACF7) using a degenerate primer-mediated PCR strategy that was optimized to res...

A microwave-assisted, one-pot, two-step protocol was developed for the construction of polysubstituted 2-aminoimidazoles. This process involves the sequential formation of imidazo[1,2-a]pyrimidinium salts from readily available 2-aminopyrimidines and alpha-bromocarbonyl compounds, followed by opening of the pyrimidine ring with hydrazine. [reaction: see text]

A two-step chromatographic procedure was developed for the isolation and purification of hen IgY antibodies from egg yolk. The antibodies were completely separated from vitellin and lipids by hydrophobic interaction chromatography followed by gel filtration. Almost no residual yolk proteins, no immunoglobulin aggregates, and no antibody fragments could be detected in the final extract. Moreover...

due to the tilt of the platform carrying the synthetic aperture radar(sar), the image formation algorithms(like rda) can not yield to good quality images from the ground. therefore, it is convenient to use a two-step approach for motion compensation in image formation algorithms. in this paper, using an approximated linear model for the motion error and the impact of range compression step of r...

Two known results in multiple testing, one relating to the directional error control of augmented step-down procedure proved by Shaffer (1980) and the other on the monotonicity of the critical values of step-up procedure proved by Dalal and Mallows (1992), are revisited and improved in this article. Shaffer’s result on the directional error control property of augmented step-down procedure with...

This paper presents a methodology for the assessment of ductility and strength capacities in low-rise buildings. This method utilizes the characteristics of force-displacement for the lowest story level or considers the weakest story in any given low-rise building for its primary analysis. Calculations are based on two levels of earthquake motions, namely strong earthquakes (PGA=0.3 g), and ver...

In this paper, we show a general way to interpret the infrastructure of a global field of arbitrary unit rank. This interpretation generalizes the prior concepts of the giant-step operation and f -representations, and makes it possible to relate the infrastructure to the (Arakelov) divisor class group of the global field. In the case of global function fields, we present results that establish ...