Celecoxib is a non-steroidal anti-inflammatory drug (NSAID) developed as a selective inhibitor of cyclooxygenase-2 (COX-2) for the treatment of rheumatoid arthritis disease. Recently some other mechanisms have been identified for anti cancer activity of these agents including induction of apoptosis, inhibition of tumor vascularization, stimulation of antitumor immune responses and inhibition of...

Aim of the study Antioxidants play an important role in maintaining physiological homeostasis. Recent literature emphasises the potential therapeutic effects of natural antioxidants that play anti-inflammatory and antioxidant effects applicable in preventing oxidative stress-induced injury, which characterises their pathogenesis. The goal of this study was to evaluate the protective role of EGC...

Compounds containing triazene ring structure are cytotoxic agents and clinically used as antitumor alkylating agents. In this study, a series of triazene derivatives holding alkyl and aryl moieties were synthesized and proved to be potent cytotoxic agents in-vitro particularly against eight cancer cell lines (PC3, HT29, Hela, HL60, Jurkat, K562, MCF7, HepG2) and a non-cancerous cell line (HUVEC...

Estradiol valerate (Exogenous estrogen) is a synthetic ester and is also a female estrogen hormone. There arestudies showing that estradiol may have anticancer effects. This study was performed to reveal the effects ofexogenous estrogens on the viability of cervical cancer cells in cell culture. We used Hela cells as our cell linein this study. Hela cells were exposed to 0.0001, 0.001, 0.01, 0....

the cytotoxic activity of ethanolic extracts of thymus vulgaris, thuja orientalis and achillea millefolium was investigated on various growing tumor cell lines. mtt colorimetric assay was used for measuring the inhibition of cell proliferation. all of the three extracts showed a relatively dose-dependent inhibition of proliferation of human breast cancer (sk-br-3, mda-mb-435) and leukemia (u937...

The HeLa cell line, named after the patient Henrietta Lacks, was one the first human cell lines to be used for tissue culture. In the decades since its origin, it has become a feature in labs across the world. Despite its longevity, repeated detection of most HeLa marker chromosomes have lead the scientific community to view HeLa as a stable cell line and its corresponding findings to be reprod...

Our previous studies, using differential mRNA display, suggested that the mouse Nip21 gene may be involved in myocarditis development in the coxsackievirus B3 (CVB3)-infected mouse heart. Sequence comparison indicated that the mouse Nip21 gene shares high sequence homology to human Nip2. This human protein is known to interact with both the apoptosis inhibitor Bcl-2 and a homologous protein, th...

Seven strains of HeLa cells have been characterized by the number of chromosomes and the activity of the enzymes alkaline phosphatase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, and lactic dehydrogenase. All seven strains were found to differ as to chromosome numbers and enzyme levels despite the fact that two strains were called HeLa and three were called HeLa S3. Thre...

The impact of radiation-induced bystander effect (RIBE) is still not well understood in radiotherapy. RIBEs are biological effects expressed by nonirradiated cells near or far from the irradiated cells. Most radiological studies on cancer have been based biochemical characterization. However, biophysical investigation with label-free techniques to analyze and compare direct irradiation RIBE has...

Poliomyelitis, an acute viral infectious disease caused by poliovirus, still remains a public health problem in developing countries. Despite the global effort to eradicate polio, continuing the polio immunization with a potent and safe vaccine is essential. For accurate vaccine evaluation, three types of cell lines including Hela, Hep2C and Vero were evaluated and compared using two methods of...