The Wilms’ tumour gene (Wt1) was originally identified as a gene involved in predisposition to the childhood kidney cancer Wilms tumour. Wt1 conventional KOs are embryonic lethal. The mutant embryos die before E13.5 days due to cardiac defects and spleen, kidney, and gonads fail to form. The role of Wt1 in haematopoiesis is controversial. Some evidence suggests that Wt1 is required for embryoni...

OBJECTIVE Two competing hypotheses can be formulated regarding the origin of canine hemangiosarcoma (HSA). One states HSA originates from differentiated vascular endothelial cells that undergo mutations which endow them with malignant potential. The other states HSA originates from transformed hemangioblastic stem cells. This study was designed to begin to distinguish between these possibilitie...

Endoglin is an accessory receptor for TGF-beta signaling and is required for normal hemangioblast, early hematopoietic, and vascular development. We have previously shown that an upstream enhancer, Eng -8, together with the promoter region, mediates robust endothelial expression yet is inactive in blood. To identify hematopoietic regulatory elements, we used array-based methods to determine chr...

We describe here conditions allowing the selective growth in culture of embryonic capillary endothelial cells from quail yolk sac. Such cultures were set up to characterize an antigen present on the endothelial cell surface and to study whether it was secreted in the culture medium. This antigen, MB1, was previously evidenced by a monoclonal antibody raised to quail IgM heavy chain. It is prese...

RATIONALE A well-developed coronary collateral circulation improves the morbidity and mortality of patients following an acute coronary occlusion. Although regenerative medicine has great potential in stimulating vascular growth in the heart, to date there have been mixed results, and the ideal cell type for this therapy has not been resolved. OBJECTIVE To generate induced vascular progenitor...