HbA1 weight in individual red cells, amounting to 13,746, was quantitatively determined in 72 male and 17 female healthy adults. The red cells were besmeared on the anti-HbA1 serum agarose plate and the precipitation rings formed were measured from the size printed on sheets of printing paper. HbA1 weight in individual cells was calculated from the calibration curve prepared specially for HbA1,...

The hemoglobins of seven species of pinnipeds-elephant, ribbon, fur, harbor, and grey seals, walrus, and California sea lion-had two major and one minor hemoglobin fractions on electrophoresis at pH 8.6. One of the major fractions in all seven species had the same electrophoretic mobility. The second fractions of the gray, harbor, and ribbon seals were similar, but different from the second fra...

BACKGROUND In Italy, diabetes centres are considered to provide adequate care but little is known about their performance. METHODS Inpatient and outpatient administrative databases were used to select and study a cohort of 2,568 diabetic patients. Adherence to guidelines and effect of patient characteristics and diabetes centre on treatment was assessed. Mortality rate was calculated. RESUL...

Aim The aim of the study is to evaluate the effect of a prospectively conducted interactive 5-day education programme based on Düsseldorf model on glycated haemoglobin (A1C), and total daily dose of insulin in type 1 diabetes patients. Methods A total of 67 type 1 diabetes patients was analysed; mean age of 11±0.68 years, 43 females and 24 males. The programme was led by a trained team of diabe...

A subtype of glycohaemoglobin, haemoglobin (Hb) A(1c), in specimens of whole blood was assayed on a new automated analyser that makes use of high-pressure liquid chromatography. The analyser provided precise and reproducible values. The mean of the HbA(1c) values was lower than that with an older instrument. The mean tended to increase with the age of the subjects, who were undergoing routine h...

A hemoglobin (hb) gene family was identified in rice, consisting of four functional hb copies located in BAC inserts OSM11719 and OSM11676, which contain the gene clusters hb1/hb3/hb4 and hb2, respectively. Sequence comparison with databases revealed that a number of potential promoters exist upstream from the hb genes suggesting that these genes are differentially expressed in rice. © 2002 Édi...

INTRODUCTION Community health workers (CHWs) can improve diabetes outcomes; however, questions remain about translating research findings into practical low-intensity models for safety-net providers. We tested the effectiveness of a home-based low-intensity CHW intervention for improving health outcomes among low-income adults with diabetes. METHODS Low-income patients with glycated hemoglobi...

The etiologic agent of chancroid is Haemophilus ducreyi. To fulfill its obligate requirement for heme, H. ducreyi uses two TonB-dependent receptors: the hemoglobin receptor (HgbA) and a receptor for free heme (TdhA). Expression of HgbA is necessary for H. ducreyi to survive and initiate disease in a human model of chancroid. In this study, we used a swine model of H. ducreyi infection to demons...

INTRODUCTION In remote Australia timely access to pathology results and subsequent follow-up of patients for treatment is very challenging due to the long distances to the nearest laboratory. Point-of-care testing (POCT) offers a practical solution for pathology service provision in such remote communities. Since 2008, POCT for haemoglobin A1c (HbA1c) has been conducted in remote Northern Terri...

We studied the influence of many variables on the cation-exchange chromatographic determination of glycohemoglobin was minicolumns (HbA1) and with macrocolumns (HbA1a+b and HbA1c). Minicolumns are most sensitive to buffer composition. The best conditions are pH 6.78 and sodium, potassium, and phosphate concentrations of 54.8, and 36 mmol/L, respectively. In fact, there is no clear cut-off point...