In this study, a number of 85 Staphylococcus aureus isolates were collected from various clinical sources including diabetic foot, burns, urine, wound, abcesses, boils, nasal discharge and vaginal smear from 135 inpatients at different Mansoura hospitals, Dakahlia governorate, Egypt. Isolates were phenotypically investigated for some virulence factors (biofilm, lipase, protease, staphylokinase ...

The hemolytic phenotype in Escherichia coli is determined by four genes. Two (hlyC and hlyA) determine the synthesis of a hemolytically active protein which is transported across the cytoplasmic membrane. The other two genes (hlyBa and hlyBb) encode two proteins which are located in the outer membrane and seem to form a specific transport system for hemolysin across the outer membrane. The prim...

Secretion of the 107-kDa hemolysin A (HlyA) from Escherichia coli is mediated by the membrane proteins hemolysin B and hemolysin D. Hemolysin B is a member of the so-called ATP binding cassette transporter superfamily, which includes the multidrug resistance P-glycoprotein, the cystic fibrosis CFTR protein, and the major histocompatibility complex-associated transporter of antigenic peptides. R...

We found that virulent hemolysin-producing (Hly+) Listeria monocytogenes strains inhibit antigen processing and presentation when added to macrophages in vitro. A virulent Hly- bacteria caused little or no inhibition. Live Hly+ bacteria inhibited presentation of both heat-killed L. monocytogenes and ovalbumin. Several observations indicate that hemolysin produced by intracellular bacteria was r...

Previous reports (Schwab, 1956a) have described the hemolytic activity of an extract of sonically disrupted group A streptococei. This is referred to as sonic extract and the hemolytic activity as intracellular hemolysin. A study comparing the hemolysin obtained by this method, with the previously described streptolysins 0 and S', indicates that the intracellular hemolysin may be a distinct str...

A multiplex PCR assay was designed to amplify the Aeromonas hydrophila and A. veronii bv. sobria hemolysin and aerolysin genes. The assay was evaluated by using 121 clinical isolates and 7 reference strains of Aeromonas spp., and these were divided into five genotypes on the basis of the results of the multiplex PCR. The five genotypes were characterized as type 1 for those carrying the ahh1 ge...

The relationship of the streptococcal hemolysin which is recognized on incubation of RBC with streptococcal cells (cell-bound hemolysin, CBH), to RNA hemolysin, a representative of oxygen-stable hemolysin (streptolysin S) has been studied. A number of similarities have been found in the conditions for optimal production of each of these hemolysins, a requirement for cysteine, Mg(++), and glucos...

Group B streptococci (GBS) are the leading cause of serious bacterial infection in newborns. Early-onset disease is heralded by pneumonia and lung injury, and the lung may serve as a portal of entry for GBS into the bloodstream. To examine a potential role for GBS beta-hemolysin in lung epithelial injury, five wild-type strains varying in beta-hemolysin expression were chosen, along with five n...

7-hemolysin, consist of two protein component, i.e,, F and S fbr leukocidin and H7I and H7II fbr 7-hemolysin, We have reported the cloning and sequencing of the leukocidin genes lukS and inkF from MRSA No. 4,2'3) In a previous report4) we purified both H7I and Hvll components to hornogeneity from S. aureus, compared their properties with those of the F and S components of leukocidin, and sugges...