S-Adenosylmethionine-homocysteine methyltransferase, which catalyzes synthesis of methionine from homocysteine, with the use of S-adenosylmethionine as the methyl donor, is absent in tumor tissue such as rat ascites hepatoma and Morris hepatoma but is present in rat liver homogenate. Absence of the enzymatic activity in tumor cells is not due to the action of an inhibitor. S-Adenosylhomocystein...

kinetics similar to those found for liver in the intact animal. This is in contrast to the lack of secretion of serum proteins by the hepatoma observed in vivo. The transfer was not inhibited when protein synthesis was interrupted by puromycin or cycloheximide or when the cells were incubated under nitrogen. U ridine, uracil, and orotic acid were efficiently incorpo rated into the RNA of hepato...

There are three hepatitis B virus (HBV) envelope glycoproteins collectively known as hepatitis B virus surface antigen (HBsAg), including the large (LHBs), middle (MHBs), and small (SHBs) surface proteins. Among them, LHBs has been retained in a large proportion of liver tissues in HBV-associated hepatocellular carcinoma (HCC) patients and shown oncogenic function in transgenic mice. However, t...

We previously reported that exosomal transfer of hepatitis C virus (HCV) positive-strand RNA from human Huh-7 hepatoma cells to human plasmacytoid dendritic cells (pDCs) triggers pDC alpha/beta interferon (IFN-α/β) production in a Toll-like receptor 7 (TLR7)-dependent, virus-independent manner. Here we show that human pDCs are also activated by a TLR7-dependent, virus-independent, exosomal RNA ...

Objective: The objective of the present study was to evaluate the cytotoxic potentials of Evolvulus alsinoides in human hepatoma HepG2 cells. Materials and Methods: HepG2 cells were treated with methanolic extract of E. alsinoides at 20, 40 and 80 µg/ml for 24 hr and cytotoxic effect was analyzed by MTT assay. The apoptosis rate was investigated by Hoechst 33342 and annexin V/propidium iodide s...

The insulin receptors in two strains of Zajdela ascites hepatoma cells have been studied. Specific binding of insulin was investigated at 4,15, and 22°,and the amount of insulin bound at steady state was proportional to the cell concentration. The optimum pH was 7.6. Scatchard analysis of the insulin binding exhibited cur vilinear plots. The number of insulin receptors was mark edly reduced in...

Survivin, an anti-apoptotic protein, is abundantly expressed in a variety of cancer cells, including hepatoma cells, resulting in the resistance of these cells to various apoptotic stimuli. Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is known to induce cancer cell-specific apoptosis, but hepatoma cells are resistant to TRAIL-induced apoptosis. In the present study, we ...

Neutral fucolipids and fucogangliosides of normal rat liver, normal rat hepatocytes, and rat hepatoma H35 and HTC cells have been compared. H35 cells were characterized by accumulation of a relatively large quantity of a few neutral fucolipids and a major fucoganglioside. HTC cells were characterized by accumulation of several neutral fucolipids and by the absence of fucogangliosides. Those fuc...

In previous studies, we observed decreased uptake of 14Clabeled t-aspartate and L-glutamate in s.c. transplants of sev eral rapidly growing hepatomas relative to that in normal liver. The present report extends these observations to isolated cells and indicates that circulation differences cannot be the major factor. Mean net uptakes for the two dicarboxylic amino acids in cells from the rapidl...

The correlation between enzyme induction and cell proliferation caused by inducers of xenobiotic metabolizing enzymes was studied using a cell culture expressing a constitutive level of cytochrome P450 (hepatoma McA RH 7777) and a cell culture in which cytochrome P450 was absent (hepatoma 27). In hepatoma 27 cells, the inducers did not induce the synthesis of xenobiotic metabolizing enzymes but...