We tested the hypotheses that the NO-cGMP-PKG pathway mediates inhibition of the store-operated cation channel (SOC) in human glomerular mesangial cells (HMC) and that TRPC4, a molecular component of SOC in HMC, is associated with PKG-phosphorylated vasodilator-stimulated phosphoprotein (VASP). Using fura 2 ratiometry, we measured intracellular Ca(2+) concentration [Ca(2+)](i) to determine whet...

Despite being successfully used to produce live offspring in many species, somatic cell nuclear transfer (NT) has had a limited applicability due to very low (>1%) live birth rate because of a high incidence of pregnancy failure, which is mainly due to placental dysfunction. Since this may be due to abnormalities in the trophectoderm (TE) cell lineage, TE cells can be a model to understand the ...

We present a variant of the HMC algorithm with mass preconditioning (Hasenbusch acceleration) and multiple time scale integration. We have tested this variant for standard Wilson fermions at β = 5.6 and at pion masses ranging from 380MeV to 680MeV. We show that in this situation its performance is comparable to the recently proposed HMC variant with domain decomposition as preconditioner. We gi...

AIM The aim of the present study was to determine the relationship between the tyrosine kinase inhibitors, STI571 and dasatinib effects and protein kinase C (PKC) status in HMC-1(560) and HMC-1(560,816) cell lines. MATERIAL AND METHODS Viability results were obtained by two different methods: MTT and a flow cytometry with Annexin V-FITC/PI double-staining protocol. The lipid-based transfectio...

Mast cells are activated by specific allergens and also by various nonspecific stimuli, which might induce physical urticaria. This study investigated the functional expression of temperature sensitive transient receptor potential vanilloid (TRPV) subfamily in the human mast cell line (HMC-1) using whole-cell patch clamp techniques. The temperature of perfusate was raised from room temperature ...

In addition to the traditional renin-angiotensin system, a great deal of evidence favors the existence of numerous independent tissue-specific renin-angiotensin systems. We report that mast cells are an additional source of renin and constitute a unique extrarenal renin-angiotensin system. We use renin-specific antibodies to demonstrate that cardiac mast cells contain renin. Extending this obse...

We present the results of an extensive infrared study of the massive star– forming region G9.62+0.19. The data cover information from broad– and narrow–band filters in the wavelength range from 1 to 19 μm and are obtained with ESO’s near– and thermal infrared camera ISAAC at the VLT and with the mid–infrared cameras TIMMI2 (La Silla, ESO) and SpectroCam-10 (Mt. Palomar). The high sensitivity an...

Hamiltonian Monte Carlo (HMC) is a powerful tool for Bayesian computation. In comparison with the traditional Metropolis–Hastings algorithm, HMC offers greater computational efficiency, especially in higher dimensional or more complex modeling situations. To most statisticians, however, idea of comes from less familiar origin, one that based on theory classical mechanics. Its implementation, ei...

This paper develops a hybrid model combining a Hidden Markov Chain (HMC) and Multilayer Perceptrons (MLP) on the Waveletheterogeneous Index of Market Shocks (WhIMS) to identify dynamically regimes in financial turbulences. The WhIMS is an aggregate measure of volatility computed at different frequencies. We estimate the model based on a French market stock index (CAC40 Index) and compare the pr...

Hydroxyurea is an effective com[»undin inhibiting the growth of a transplantable mammary tumor (HMC) in the Fischer rat, the inhibition being dose related and independent of the duration of treatment. Several compounds related to hydroxyurea were tested and compared to the parent compound. 1-Ethyl-lhydroxy urea and 1-methyl-l-hydroxyurea are more effective than hydroxyurea in causing inhibitio...