Fordiae Cauliflorae Radix (FC, the root of Fordia cauliflora Hemsl) and Millettiae Pulchrae Radix [MP, the root of Millettia pulchra (Benth.) Kurz var. laxior (Dunn) Z. Wei], which go under the same local name of "Daluosan", have long been used in Southern China for the treatment of stroke, paralysis, dementia in children, Alzheimer's disease and other diseases. The same local name and similar ...

Moutan Cortex (MC) has been demonstrated to have an inhibitive effect on inflammation and oxidative stress responses in mesangial cells in our previous study. However, little is known about the components of MC contributing to this benefit. In the present study, cell membrane immobilized chromatography (CMC), a fast and useful method, was presented for screening potential active components of M...

The antioxidant activity and phenolic composition of six in vitro cultured blueberry seedlings were determined. Extracts were prepared in 85% ethanol from 30 days old in vitro cultured plants and used to evaluate the antioxidant capacities that included Ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazin (DPPH•) scavenging ability, total polyphenols (TP) and the partial p...

The development of novel antibacterial drugs needs urgent action due to the global emergence antibiotic resistance. In this challenge, actinobacterial strains from arid ecosystems are proving be promising sources new bioactive metabolites. identified Streptomyces rochei strain CMB47, isolated coal mine Saharan soil, provided an ethyl acetate extract which tested against a series pathogens. It d...

Optimization of sample pre-treatment. Complete removal of cellular and further solid sample constituents, precipitation and removal of proteins, precipitation and removal of cholesterol from the blood sample and quantitative extraction of the analytes tryptophan, phenol, p-cresol and cholic acid in the diluted blood plasma phase. Step III: Combination and conversion of both isocratic HPLC/DAD/M...

The aim of this work was to characterise the phenolic compounds in artichoke (hearts) by using HPLC coupled to DAD-ESI-QTOF-MS, which proved useful in characterising 61 phenolic and other polar compounds. Notably, of the 61 compounds characterised, 34 new phenolic compounds with their isomers have been tentatively characterised in artichoke for the first time, namely: 3 hydroxybenzoic acids, 17...

A rapid, sensitive extraction method was developed using the mixture MethanolDichloromethane-Water (MDW) (0.3:4:1v/v/v) and MeOH-H2O phase was assayed for sugar analysis. Photodiode-array detection (DAD) has been used to prove the extracted compound is UV inactive, High-performance liquid chromatography (HPLC) with Evaporative Light Scattering Detector (ELSD) coupled to electrospray ionization ...

This study falls within the framework of the industrial exploitation of by-products of the prickly pear (Opuntia ficus-indica). The study aims to evaluate the use of hydro-ethanolic extract of prickly pear peels as a substitute of vitamin E used as antioxidant in margarine preservation. The extract was rich in total phenolics (1512.58 mg GAE/100 g DM). HPLC-DAD-ESI-MS(n) analyses allowed the id...

A validated HPLC-DAD-ESI-MS(n) method for the analysis of non-anthocyanin flavonoids was applied to nine different tissues of twelve lotus genotypes of Nelumbo nucifera and N. lutea, together with an optimized anthocyanin extraction and separation protocol for lotus petals. A total of five anthocyanins and twenty non-anthocyanin flavonoids was identified and quantified. Flavonoid contents and c...

A determinação qualitativa e quantitativa de carotenóides pode fornecer diferentes e importantes informações sobre os organismos que os contêm. Na análise de pigmentos por HPLC diversos detectores podem ser utilizados, como diode array (DAD) e eletroquímico (ED). O presente trabalho tem como objetivo desenvolver um método por HPLC utilizando uma coluna C 30 para a identificação e quantificação ...