Background: To develop the high-performance thin layer chromatography (HPTLC) finger print profile of hydroalcoholic and ethylalcohol extracts seeds Tudri Surkh (Cheiranthus cheiri). Methods: Chromatographic technique was used for separation components from HPTLC carried out using CAMAG system equipped with Linomat V applicator, WinCATS software.Results: profiling extract confirm presence vario...

Abstract Two solvent mixtures for high-performance thin-layer chromatographic (HPTLC) separation of some compounds showing estrogenic activity in the yeast estrogen screen (YES) assay are presented. The new method, planar (pYES) combines YES and a on silica gel HPTLC plates with performance assay. For separation, analytes were applied bandwise to (10 × 20 cm) fluorescent dye (Merck, Germany). d...

The objective of the present work was to develop a simple, specific, and fast high-performance thin-layer chromatographic (HPTLC) method identify quantify cordifolioside A, 20-β-hydroxyecdysone columbin with HPTLC‒electrospray ionization‒tandem mass spectrometry (ESI‒MS/MS) for characterization in Tinospora cordifolia stem extracts. Chromatographic development performed using HPTLC aluminum pla...

In most cases the pharmacological activity of plant extracts is not assigned to single components and often not all active ingredients are known. Approaches other than those considering single compounds only to analyze plant material have proven helpful for a better characterization of extracts in their entirety. In this study extracts of willow bark are analyzed by high-performance thin-layer ...

Most of the traditional medicinal plants in India are not scientifically validated. Scientific evaluation along with traditional knowledge is essential to obtain effective drugs for commercial purpose. Solena amplexicaulis belongs to the family, Cucurbitaceae, a traditional medicinal plant species of Tamil Nadu, India is being prescribed to cure various diseases. The present study is to establi...

Pteris vittata L., a fern was evaluated for its alpha amylase inhibitory potential owing to its previously reported antihyperglycemic activity. This Pteridophyte was also assayed for its free radical inhibition ability in ABTS decolourisation assay followed by quantification and HPTLC of polyphenols. The Porcine pancreatic alpha amylase inhibition was studied invitro and compared with standard ...

A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of valsartan and hydrochlorothiazide in tablet formulations. Standard and sample solutions of valsartan and hydrochlorothiazide were applied to precoated silica gel G 60 F254 HPTLC plates and the plates were developed with chloroform–ethyl acetate–acetic...

We have developed a simplified and improved high performance liquid chromatography (HPLC) method for the detection and quantitation of tissue lipid using a new laser light-scattering detector (Varex model ELSD II). This detector has a limit of sensitivity of 50 ng for neutral lipid and 200 ng for most phospholipids with excellent reproducibility. By coupling the ELSD II with a ternary gradient ...

Ginger rhizome powder is widely used as a food spice and an herbal medicine around the world. A sensitive and accurate High Performance thin layer chromatographic (HPTLC) method has been developed to determine the quantity of 10-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams on aluminum-backed silica gel 60 F254 plates with n-hexane...

High performance thin layer chromatography (HPTLC) was used to determine the phospholipid and sphingolipid profiles in the feces of BALB/c mice infected with Echinostoma caproni (Trematoda) versus uninfected controls. Lipids extracted from fecal samples with chloroform-methanol, 2:1, were separated on silica gel HPTLC plates developed in chloroformmethanol-water, 65:25:4. Densitometric analysis...