the interaction of herpes simplex virus type 1 (hsv-1) with human fetal foreskin fibroblast (hfff) cell was studied using a recent isolate of hsv-1 which was propagated in hep-2 cells. hfff cells were challenged with hsv-1 with a multiplicity of infection (moi) of 1 virus/cell for 24 hours. flow cytometric analysis demonstrated that hsv-1 challenged hfff cells expressed increased levels of α2β1...

Methods for the selective enrichment of various subpopulations of the human skin fibroblast cell line HH-8 have been developed. These methods permit the selection of homogeneous populations of the three mitotic fibroblast cell types MF I, II and III, and the four postmitotic cell types PMF IV, V, VI and VII. These seven cell types exhibit differentiation-dependent and cell-type-specific pattern...

The aim of this study was to investigate the antioxidant properties root extract Onosma armeniacum and its cytotoxic effect against human liver (HepG2), lung (A549) colon (WiDr) cancer cell lines compared with a normal fibroblast line. Antioxidant determined using colorimetric methods. were investigated MTT assay. total phenolic content reducing power values 26.1±0.4 mg gallic acid equivalent 5...

The cytotoxicity of amorphous calcium phosphate (ACP) and chitosan lactate (ChOL) multifunctional hybrid composite coatings on MRC-5 human lung fibroblast cell line was elucidated. ACP/TiO2 ACP/TiO2/ /ChOL were deposited onto Ti by a novel in situ anodization/anaphoretic process at constant voltage. Cytotoxicity tests showed that there no significant decrease the survival healthy cells exposed ...

abstractferulago angulata boiss. known in iran as chavir, has some bioactive compounds having antioxidant activity. because of its antioxidant activities, it sounded chavir extract can be a good candidate for finding chemopreventive agents having inductive apoptosis properties on cancer cells. in this study, the cytotoxic effects and proapoptotic activities of chavir’s leaf and flower extracts ...

The objective of the study was to evaluate the cytotoxic effects of aqueous extract of Cucumis melo fruit on human prostate cancer cell line (PC-3) using MTT and neutral red assays. The crude aqueous extract of C. melo was prepared by cold maceration method, filtered, concentrated and tested on PC-3 cell line. Dose-dependent cytotoxic activities were exhibited on human prostate carcinoma PC...

The objective of the study was to evaluate thecytotoxic effects of aqueous fruit extract of Cucumis melo in human prostate cancer cell line (PC-3) using MTTand neutral red assays. The crude aqueous extract of C.melo was prepared by cold maceration method, filtered,concentrated and tested on PC-3 cell line. Dose-dependent cytotoxic activitieswere exhibited by human prostate carcinoma PC-3 cell l...

transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. they have a growth inhibitory role on hemopoietic progenitor cells in semisolid colony assay as well as in long-term bone-marrow culture. tgf - β2 represses stromal cells, stem cell factor gene transcription, and decreases the stability of c-kit transcripts in hemopoietic cells. tgf-β also modulates g...

Abstract: Background  and  Aims:  Huh-7  is  a  cell  line  that  was  derived  from  a  liver  tumor  of  a  Japanese  man.  Hepatocellular  carcinoma  (HCC)  is  considered  as  a  primary  liver  cancer.  Highly  resistant  tumor  to  treatment  which  causes  the  death  of  many  patients  annually.  Thus,  targeting  the  cancer  cells  by  using  a  new  method  could  be  effective  in...

Recently, there has been a great interest in melanoma and stromal cell-to-cell interactions behind the progressive behavior of melanoma. By vitro co-culturing using sequential seeding conditions imitating niche properties, gained hybrid variants are visualized by high content fluorescent microscopy. The co-culture experiment applied consecutive order primarily plated HDF-RFP-ZeoR fibroblast mon...