The phosphorylation of purified protein synthesis factors catalyzed by protein kinase preparations isolated from interferon-treated human amnion cells was examined. Ribosomal salt-wash fractions prepared from interferon-treated human cells contained a protein kinase that catalyzed the [gamma-(32)P]ATP-mediated phosphorylation of the 38,000-dalton subunit of eukaryotic initiation factor 2 (eIF-2...

An Rc-mutant of Escherichia coli that lacks UDPgalactose 4-epimerase grows normally without galactose but makes lipopolysaccharide lacking most of its carbohydrate. Exogenous galactose overrides the mutation and results in the formation of a complete lipopolysaccharide, thereby producing a smooth phenocopy. The smooth phenocopy was much more resistant to the bactericidal activity of normal huma...

PHARMACOLOGY AND TOXICOLOGY T HE PHARMACOLOGY of interferons has been studied with human leukocyte interferon and with mouse and rabbit interferons. These have a short half-life in the blood, irrespective of the route of administration, though the most rapid clearance follows the intravenous route; intramuscular injections result in peak blood levels after 2-4 hr, lasting for 4-6 hr. With intra...