Five patients treated with intraperitoneal 131I-labeled mouse monoclonal antibody for ovarian cancer also received i.v. exogenous polyclonal human anti-murine immunoglobulin antibody. The pharmacokinetics of 131I-labeled monoclonal antibody in these patients were compared with those of 28 other patients receiving i.p.-radiolabeled monoclonal antibody for the first time without exogenous human a...

Using a polyclonal antibody against human epidermal keratins and a monoclonal antibody against cytokeratins characteristic of simple epithelia, and the Avidin-Biotin system of immunohistochemistry, we have demonstrated cytokeratin expression in 46% and in 60% of small cell carcinomas of the lung at autopsy respectively. The latter gave a diffuse stronger reaction product than the polyclonal ant...

background: during the last twenty years, the extraction of specific egg yolk (igy) antibodies from the immunized chickens has been accepted as a useful alternative to the immunization of mammals. the aim of the present study was immunizing the chickens with human umbilical cord serum (hucs) and the extraction of specific anti-human globulins (igg, c3b, and c3d) antibodies from egg yolk in orde...

Submit Manuscript | http://medcraveonline.com Abbreviations: SPR: Surface plasmon resonance; ISFET: Ion Sensitive Field Effect Transistor; EnFET: Enzyme Field Effect Transistor; μTAS: Micro Total Analysis System; HCMV: Human Cytomegalovirus; ELISA: Enzyme-Linked Immunosorbent Assay; CNC: Computer Numerical Control; CAD: Computer Assisted Design; BSA: Bovine Serum Albumin; HPAb: Human Polyclonal...

Methods. Frozen sections obtained from eight human autopsy eyes were placed on gelatincoated slides. After blocking of nonspecific binding sites, the slides were incubated with rabbit polyclonal antibody to the latency-associated peptide (LAP) region of human TGF-01, TGF02, and TGF-/33 precursors, followed by the incubation with biotinylated swine anti-rabbit IgG. Subsequently, a streptavidin-l...

A highly specific and reproducible antigen-induced, antigen-specific culture and assay system for antibody production by human peripheral blood B lymphocytes has been developed. The system is clearly T cell and monocyte dependent and is independent of exogenous mitogens. The major factors in our ability to trigger specific antibody production with antigen alone have been the use of extremely lo...

Cellular receptors play an important role in virus pathogenesis. As a first step in virus infection, viruses attach to specific receptors on the surface of cells; Hantaan virus infects susceptible cells by attaching to a receptor located on the cell surface. To date, the identity of the Hantaan virus host cell receptor remains unknown. To determine the protein on the cell surface to which Hanta...

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...

In order to develop an indirect competitive enzyme-linked immunosorbent assay (ELISA) for the insecticide imidacloprid, the anti-imidacloprid polyclonal antibody was produced and the affinity of three different coating antigens with the polyclonal antibody was compared. The antigenic determinant in antigen aiming at the polyclonal antibody was raised and analyzed. The standard curve for imidacl...

A murine hybridoma cell line that produces a monoclonal IgG1 antibody to human factor IX was established to provide a conformational probe for the clotting factor and its genetic variants. The antibody inhibited factor IX procoagulant activity, but did not appreciably interfere with the cleavage of factor IX by factor XIa nor with the binding of antithrombin-III-heparin complex to factor IXa. T...