PURPOSE To investigate the cellular effects of mitomycin C (MMC) treatment on corneal endothelial (CE) cells at clinically relevant applications and dosages. METHODS Radial and posterior diffusion of MMC was determined by an Escherichia coli growth inhibition bioassay. A modified version of the comet assay (single cell gel electrophoresis) was used to detect DNA cross-linking. Immunostaining ...

ABSORPTION AND RELEASE OF MITOMYCIN-C BY “SPONGOSTAN FILM”. LARROSA JMl, FERRER J2, PINILLA 11, POLO VI, SANCHEZ Al, BUENO J’, HONRUBIA FMl. 1 Department of Ophthalmology, “Miguel Set-vet” Hospital. Zaragoza,(Spain) 2 Department of Microbiology, Puroose: To determine the Mitomycin-C (MMC) 0,4 mg./ml. absorption of spumous specimens of “Spongostan Film” (“Sp”) and their liberation rate. Methods:...

Glutathione (GSH) has been shown to modulate the cytotoxicity of a variety of chemotherapeutic agents. The effect of mitomycin C (MMC) treatment duration and the effect of GSH depletion on in vitro cytotoxicity against the human colon cancer cell line HT-29 was studied under aerobic conditions. Continuous-exposure experiments revealed that the cytotoxicity of 0.1 microM MMC, as measured by clon...

We developed a Mitomycin C (MMC)-resistant mouse breast cancer cell subline, R-FM3A, from FM3A parent cells(W-FM3A) by continuous exposure to a concentration of 0.01 microgram/ml MMC for over two months. R-FM3A cells were resistant to MMC and decreased in response to the combined effects of ionizing irradiation and MMC. The resistance to MMC and ionizing irradiation was mediated by a cell-free ...

In the medium voltage direct current (MVDC) transmission system, a small number of MMC sub modules will reduce power quality. this paper, based on research background Photovoltaic Medium Voltage Direct Current (PV-MVDC) Hybrid Modulation Strategy Decoupled Double Synchronous Reference Frame (DDSRF) control strategy is proposed. The dual arm complementary hybrid modulation combining nearest-leve...

ABSORPTION AND RELEASE OF MITOMYCIN-C BY “SPONGOSTAN FILM”. LARROSA JMl, FERRER J2, PINILLA 11, POLO VI, SANCHEZ Al, BUENO J’, HONRUBIA FMl. 1 Department of Ophthalmology, “Miguel Set-vet” Hospital. Zaragoza,(Spain) 2 Department of Microbiology, Puroose: To determine the Mitomycin-C (MMC) 0,4 mg./ml. absorption of spumous specimens of “Spongostan Film” (“Sp”) and their liberation rate. Methods:...

AIM To compare 1-year follow-up results of photorefractive keratectomy (PRK) with mitomycin C (MMC) and laser in situ keratomileusis (LASIK) for custom correction of myopia. METHODS Eighty-eight eyes of 44 patients with moderate myopia were randomised to PRK with 0.002% MMC for 1 min in one eye and LASIK in the fellow eye. The 1-year follow-up was evaluated. RESULTS There were no difference...

The effect of chemotherapy drug Mitomycin C (MMC) in combination with recombinant adeno-associated virus II (rAAV2) in cancer therapy was investigated, and the mechanism of MMC affecting rAAV2's bioactivity was also studied. The combination effect was evaluated by the level of GFP and TNF expression in a human glioma cell line, and the mechanism of MMC effects on rAAV mediated gene expression w...