This paper presents a highly efficient single cell capture scheme using hydrodynamic guiding structures in a microwell array. The implemented structure has a capturing efficiency of >80%, and has a capacity to place individual cells into separated microwells, allowing for the time-lapse monitoring on single cell behavior. Feasibility was tested by injecting microbeads (15 μm in diameter) and pr...

A theoretical model based on the molecular interactions between a growing tumor and a dynamically evolving blood vessel network describes the transformation of the regular vasculature in normal tissues into a highly inhomogeneous tumor specific capillary network. The emerging morphology, characterized by the compartmentalization of the tumor into several regions differing in vessel density, dia...

Fabricated solid-state nanopore chips are used to probe individual single stranded DNA (ssDNA) homopolymers. Based on the analysis of the current blockage caused by DNA translocation through a voltage-biased nanopore, we discovered that the hydrodynamic diameter of ssDNA homopolymer helix is comparable to that of double stranded DNA (dsDNA) helix. This proof-of-principle demonstration shows tha...

The hydrodynamic coupling between two isolated 0.97 mm diameter polystyrene spheres is measured by reconstructing their Brownian motion using digital video microscopy. Blinking optical tweezers are used to facilitate data collection by positioning the spheres in the microscope’s focal plane and in close proximity to one another. The observed separation dependence of the spheres’ relative and ce...

We demonstrate ElectroWetting-On-Dielectric (EWOD) transport and SQUID gradiometer detection of magnetic nanoparticles (MNPs) suspended in a 2 microl de-ionized water droplet. This proof-of-concept methodology constitutes the first development step towards a highly sensitive magnetic immunoassay platform with SQUID readout and droplet-based sample handling. Magnetic AC-susceptibility measuremen...

A study of the temperature-dependent solvation dynamics of coumarin-480 in [bmim][BF4]/Triton X-100/cyclohexane microemulsions at three different temperatures (299 K, 308 K and 317 K) is reported. The solvation dynamics of a room temperature ionic liquid in microemulsions becomes faster with increase in temperature. Dynamic light scattering measurements indicate a significant change in size wit...

We use molecular dynamics simulations to study the crystallization of spherical nucleic-acid (SNA) gold nanoparticle conjugates, guided by sequence-specific DNA hybridization events. Binary mixtures of SNA gold nanoparticle conjugates (inorganic core diameter in the 8-15 nm range) are shown to assemble into BCC, CsCl, AlB(2), and Cr(3)Si crystalline structures, depending upon particle stoichiom...

This review provides a brief overview of particle size characterization by Ž . Sedimentationr Steric Field-Flow Fractionation SdrSt FFF and discusses the retention in steric FFF, the recent findings on hydrodynamic lift forces, and the density compensation calibration procedure for obtaining size distribution of supramicron-sized particles. A number of applications to the determination of parti...

The data presented here are related to the research paper entitled "PCL-b-P(MMA-co-DMAEMA)2 new triblock copolymer for novel pH-sensitive nanocapsules intended for drug delivery to tumors" by Franco et al. [1]. Characterization data of PCL-diol, macroinitiator Br-PCL-Br, homopolymers (PMMA and PDMAEMA) and copolymers (batch 1 and batch 2) analyzed by FTIR, SEC and NMR, as well as, characterizat...

A simple low temperature colloid-chemical synthetic method is reported for size controlled synthesis of hydrophobic silicon nanoparticles in the 1-10 nm range. These silicon nanoparticles show size dependent tunable visible emission from blue to red with fluorescence quantum yield in the range of 6-13%. These silicon nanoparticles can be subjected to extensive surface chemistry without signific...