A real time monitoring of fouling in liquid chromatography has been presented. The versatility of the approach has been proven by successful implementation in three case studies with an error <1%. The first application demonstrates the monitoring of protein A ligand density and foulant concentration for assessing performance of protein A chromatography resin during purification of monoclonal an...

This review summarizes the use of computer assisted liquid chromatographic method development for the analytical characterization of protein biopharmaceuticals. Several modes of chromatography including reversed-phase liquid chromatography (RPLC), ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC) and some perspectives are discussed. For all these chromatographic mo...

pluronic/bile salt/phospholipid mixed micelles (pluronic/bs/ps-mm) drug carrier system for solubilization hydrophobic drugs was developed. a typical hydrophobic compound, pyrene, was selected as a representative hydrophobic compound to model the hydrophobic drugs. five pluronics, f68, f88, f98, f108, and f127 with different ppo chain length were studied. cmc data and solubilization capacities w...

A procedure has been developed for purification of the tripartite anthrax-toxin components. This involves sequential high-performance anion-exchange, gel-filtration and hydrophobic-interaction chromatography. From an initial culture volume of 15 litres, typical yields of 8 mg of protective antigen, 13 mg of lethal factor and 7 mg of oedema factor are produced to higher degrees of purity than ha...