THE DETERMINATION of hydroxyproline in parenchymous tissues such as liver presents special problems not encountered in collagenons tissues. This is true because liver contains only small quantities of this imino acid (1), and furthermore, the existing quantitative tests are nonspecific. Thus, if one were to use the usually acceptable Neuman and Logan procedure (2), a separate determination for ...

The aim of the research is a qualitative and quantitative study amino acid composition Syrian rue (Peganum harmala, Nitrariaceae), growing in natural conditions Azerbaijan Republic. After preliminary confirmation presence acids, plant parts were subjected to in-depth by ion-exchange chromatography using post-column derivatization on L-8800 analyzer (Hitachi, Ltd.). Amino analysis roots P. condu...

Mature male Sprague-Dawley rats were subjected to an isoproterenol-induced myocardial infarction. Animals were sacrificed on a daily basis in order to assess the temporal changes in prolyl hydroxylase activity and collagen metabolism during the acute stages of myocardial necrosis and repair. Total myocardial hydroxyproline, as an indexof collagen content, increased promptly and markedly, beginn...

Iminodipeptides containing C-terminal proline or hydroxyproline were determined in sera from patients with prolidase deficiency, in their mother's serum, and in the sera of unrelated controls, using liquid chromatography-mass spectrometry with an atmospheric pressure ionization interface system. The separation was carried out on a reversed phase column using 1 g/l aqueous trifluoroacetic acid-m...

hydroxyproline level when the alkaline phosphatase level is of the order found in this patient. Further evidence of normal bone metabolism was obtained from the normal hydroxyproline excretion. There was no laboratory evidence to suggest hyperparathyroidism. The normal galatose-l-phosphate uridyl transferase level ruled out galactosaemia. Though a diagnosis of glycogen storage disease was consi...

objective: to validate the ethno-therapeutic claim of the traditionally used plant jasminum auriculatum (j. auriculatum) in skin diseases, by evaluating its wound healing potential along with its antioxidant and antimicrobial properties; so as to understand their role in wound healing. materials and methods: excision and incision wound models were used to evaluate the wound healing activity on ...

Hydroxyproline Z-epimerase, purified to homogeneity from hydroxyproline-induced cells of Pseudomonas pufida, behaved like a single peptide chain of approximately 64,000 molecular weight, based both on ultracentrifugal data and on a study of tryptic peptides and amino acid composition. Several methods indicated a total of 12 reduced cysteines per mole of native enzyme. Of these only six reacted ...