Indole-3-acetic acid (IAA) homeostasis was investigated during seed germination and early seedling growth in Scots pine (Pinus sylvestris). IAA-ester conjugates were initially hydrolyzed in the seed to yield a peak of free IAA prior to initiation of root elongation. Developmental regulation of IAA synthesis was observed, with tryptophan-dependent synthesis being initiated around 4 d and tryptop...

A novel piezoelectric immunosensor has been developed for the determination of beta-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antige...

The seasonal change of free abscisic acid (ABA) and indole-3-acetic acid (IAA) and their relationship with the cambial activity in Eucommia ulmoides trees were investigated by ABA and IAA immunolocalization using primary polyclonal and rhodamine-red fluorescing secondary antibodies, ABA and IAA quantification using high performance liquid chromatography (HPLC), and systematic monitoring of vasc...

An important process for the regulation of auxin levels in plants is the inactivation of indole-3-acetic acid (IAA) by conjugation to amino acids. The conjugation reaction is catalysed by IAA-amido synthetases belonging to the family of GH3 proteins. Genetic approaches to study the biological significance of these enzymes have been hampered by large gene numbers and a high degree of functional ...

The plant hormone auxin (IAA) coordinates many of the important processes in plant development. For example, IAA is critical for normal embryogenesis, root development, cell elongation, and the tropic responses such as gravitropism and phototropism. IAA gradients are established and maintained in many tissues and it is thought that these gradients act as developmental cues, determining the fate...

Results Overall, 1790 patients showed no post-operative complications and were assigned to the non-complication group, whereas 27 patients suffered from IAA postoperatively and were assigned to the IAA group. The incidence of IAA after LA was 1.4% and the only identified risk factor for IAA was dirty fluid collection in the peritoneal cavity observed during the operation. (P < 0.001) On logisti...

The plant hormone auxin can be regulated by formation and hydrolysis of amide-linked indole-3-acetic acid (IAA) conjugates. Here, we report the characterization of the dominant Arabidopsis iaa-leucine resistant3 (ilr3-1) mutant, which has reduced sensitivity to IAA-Leu and IAA-Phe, while retaining wild-type responses to free IAA. The gene defective in ilr3-1 encodes a basic helix-loop-helix leu...

Indoleacetic acid (IAA) oxidase has been reported to be involved in plant growth because of its alleged role in the control of endogenous IAA levels. This purported role was reevaluated in terms of the properties and subcellular location of the enzyme in etiolated pea (Pisum sativum L. var. Alaska) epicotyls.The enzymic properties of IAA oxidase in the floating pea epicotyl segments are similar...

Effect of growth hormones i.e., gibberellic acid (GA3), indole-3-acetic acid (IAA) and Kinetin was studied on the root of Cicer arietinum L. after forty-five and sixty days of treatments and compared with control. The application of GA3 alone and in combination with IAA or Kinetin or both caused extension growth and insignificant increase in the number of rootlets accompanied with decrease in t...

The endogenous levels of indole-3-acetic acid (IAA) and phenyl acetic acid (PAA) were estimated in developing seeds of two different varieties of Cajanus cajan: Black seeded variety (V1, big seeded) and B. D. N2 (V2, small seeded). Antibodies against IAA and PAA were raised in rabbits. By competitive ELISA, free and conjugated IAA and free forms of PAA were estimated. Growth analysis of seeds w...