IL-4 receptor alpha chain (IL-4Ralpha)-deficient mice were generated by gene-targeting in BALB/c embryonic stem cells. Mutant mice showed a loss of IL-4 signal transduction and functional activity. The lack of IL-4Ralpha resulted in markedly diminished, but not absent, TH2 responses after infection with the helminthic parasite Nippostrongylus brasiliensis. CD4+, CD62L-high, and CD62L-low T cell...

To observe the pro- and anti-inflammatory cytokine (TNF-α, IL-4, IL-6, IL-10) secretion from cultivated peripheral blood mononuclear cells (PBMCs) for 48h. Blood samples were obtained 42 women patients of Nadezhda Women’s Health Hospital after signing written informed consent. For PBMC isolation, 9 ml heparinised whole each patient processed by density gradient centrifugation using Pancoll (P04...

Introduction: Multiple sclerosis is a chronic inflammatory disease of central nervous system. The etiology of MS is slightly known, but genetic and environmental factors are reported. Vitamin D regulates gene expression and affects target cell functions. The aim of this study was to investigate the expression variation of IL-2 and IL-4 genes under vitamin D supplementation in patients with mult...