BACKGROUND Pregnancy represents an exclusive situation in which the immune and the endocrine system cooperate to prevent rejection of the embryo by the maternal immune system. While immature dendritic cells (iDC) in the early pregnancy decidua presumably contribute to the establishment of peripheral tolerance, glycoprotein-hormones of the transforming growth factor beta (TGF-beta) family includ...

BACKGROUND In this review, recent advances in the laboratory as well as embryological aspects of hCG priming in vitro maturation (IVM) cycles are described. METHODS This report is based on publications from literature searches and the authors' experience. RESULTS In IVM cycles, priming with hCG permits the recovery of a certain number of oocytes with an expanding/dispersed cumulus pattern w...

Attempts were made during the 1990 growing season to induce somatic embryos from non-zygotic tissues of five economically important walnut cultivars. The following explants were employed: fertilized and unfertilized ovules; immature and mature catkins; abnormal "spike" flowers; and in vitro-grown leaves and roots. Modifications of the standard induction media were also examined. These included ...

mdash; Plant regeneration from immature intergeneric embryos through direct callus induction was investigated in the case of hybridization between T aestivum cv Chinese Spring (2n = 6x = 42, AABBDD) and A cristatum 4x with one B chromosome (2n = 4x = 28 + 1 B, PPPP). Twenty-three abnormal immature hybrid embryos were directly cultured on MS solid medium with 2 mg / L 2,4-D to induce calli which...

Introduction. To report a live birth following egg retrieval after only 12 hours from hCG priming. Patients. A childless couple with five-years-lasting secondary infertility. Methods. IVF was performed according to the long protocol. Two immature oocytes were retrieved following only 12 hours after hCG priming due to the patient misunderstanding. The eggs were cultured in vitro and ICSI was per...

MAHMOOD, I., A. RAZZAQ, M. RASHEED, A. QAYYUM and M. AHMAD, 2014. Employment of immature embryo culture for in vitro selection of drought tolerant somaclones of wheat. Bulg. J. Agric. Sci., 20: 155-161 Drought, a serious threat to world agriculture, demands neo-breeding approaches. Tissue culture being a mutagenic process induces somaclonal variations, which can be manipulated for improving dro...

This article presents the results of research on production mutant doubled haploids turnip rape (Brassica rapa L.ssp.Oleifera), as well its interspecies hybrids with rapeseed napus). An  immature embryo culture  was used to increase productivity inter-specific hybridization. Studies have obtained interspecific rapeseed. The largest number plants observed in where mother plant Furtherm...

Following in vitro maturation, approximately 90% of immature bovine oocytes will reach metaphase II and extrude the first polar body; approximately 80% will undergo fertilization and cleave, at least once, to the two-cell stage. However, only about 30-40% will ever reach the blastocyst stage. This would suggest that the post-fertilization part of the process of in vitro embryo production, the l...

The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor ...

The aim of this study was to evaluate the suitability of a commercial kit for bovine embryo vitrification for cryopreserving cat oocytes and to evaluate comparatively the effects of its use with slow freezing procedure on cryotolerance in terms of morphology and oocyte resumption of meiosis. Germinal vesicle stage oocytes isolated from cat ovaries were either vitrified (n = 72) using a vitrific...