there are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals edcs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. the yolk protein precursor vitellogenin (vtg) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to edcs especially...

in this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.then, the cells were cultivated as monolayer in mem (minimum essential medium eagle). the culture mediawere collected after 72 h and frozen at -20°c. carp ovarian follicles also were separated mechanically andincubated in bss (basic salt solution) cortland medium in 24-well microplates for 48 h...

A new enzyme immunoassay (Abbott ER-EIA Monoclonal) for the determination of estrogen receptor in cytosols from breast tumor specimens has been developed by Abbott Laboratories. To establish the correlation of the results from this new technique with currently existing steroid binding methods, a multicenter study was conducted in eight European laboratories. All participants followed the same p...

A centrifugal compact disc (CD) microfluidic platform with reservoirs, micro-channels, and valves can be employed for implementing a complete immunoassay. Detection or biosensor chambers are either coated for immuno-interaction or a biosensor chip is inserted in them. On microfluidic CDs featuring such multi-step chemical/biological processes, the biosensor chamber must be repeatedly filled wit...

BACKGROUND The measurement of 25OH vitamin D continues to grow in clinical laboratories. The aim of this multi-center study was to compare the results of seven automated commercial immunoassays with a reference HPLC technique. METHODS One hundred and twenty consecutive outpatient serum samples were centrifuged, divided in aliquots, frozen and shipped to the participating laboratories. 25OH Vi...

Aflatoxin B I (AFB) isa well known hepatocarcinogen in several animal species and probably a causative agent in human hepatocellular carcinoma. Humans are exposed to AFB by ingesting contaminated food. Aflatoxin contamination encountered in human foods is usually at low levels which is difficult to measure by chromatographic methods. Therefore in the present study we have developed an immun...