Influenza viruses cause significant morbidity and mortality in both children and adults during local outbreaks or epidemics. Therefore, a rapid test for influenza A&B would be useful. This study was conducted to evaluate the clinical performance of the Wondfo influenza A&B test for rapid diagnosis of influenza A H1N1 Infection. The rapid testing assay could distinguish infection of influenza A ...

Cannabis sativa L. is one of the most consumed drugs in world and recent studies have associated its use with an increase number traffic accidents different countries. In many countries, like Brazil, simple reliable methodologies are still needed for detection on site, mainly cannabinoids, considering prevalence oral fluid (OF) has been proved as appropriate biological matrix this purpose. Cons...

The recent Zika virus (ZIKV) outbreak demonstrates that cost-effective clinical diagnostics are urgently needed to detect and distinguish viral infections to improve patient care. Unlike dengue virus (DENV), ZIKV infections during pregnancy correlate with severe birth defects, including microcephaly and neurological disorders. Because ZIKV and DENV are related flaviviruses, their homologous pro...

Cervical cancer is still become main health problem especially in development country. This the second most common Indonesian women. It commonly caused by infection of Human Papillomavirus (HPV) type 16 and 18. Actually if cervical was detected earlier, it shows better respond therapy than other cancers, but this opportunity not supported appropiate screening methods. Nowadays, pap smear IVA ar...

We have recently treated with N2 gas plasma and achieved inactivation of bacteria. However, the effect of N2 gas plasma on viruses remains unclear. With the aim of developing this technique, we analyzed the virucidal effect of N2 gas plasma on influenza virus and its influence on the viral components. We treated influenza virus particles with inert N2 gas plasma (1.5 kpps; kilo pulses per secon...

Current trends in the development of on-site diagnostic tests using immunodetection principles are proceeding in two directions: toward the immunochromatography test strip and toward the immunosensor. To develop both applications for the detection of digoxin, we prepared multilabeled antibody conjugates using colloidal gold and ferrocene. Prior to applying the digoxin antibody in immunochromato...

The aim of this study was to make the first report on canine heartworm disease in the state of Rondônia and confirm its transmission in this state. Blood samples were randomly collected from 727 dogs in the city of Porto Velho. The samples were analyzed to search for microfilariae and circulating antigens, using three different techniques: optical microscopy on thick blood smears stained with G...

PURPOSE There is a need for new technologies to study tissue-based biomarkers. The current gold standard, immunohistochemistry, is compromised by variability in tissue processing and observer bias. Reverse transcription-PCR (RT-PCR), immunocytochemistry, and reverse-phase lysate microarrays (RPM) are promising alternative technologies but have not yet been validated, or correlated, on the same ...