We analysed the import pathway of Tim23 and of Tim17, components of the mitochondrial import machinery for matrix-targeted preproteins. Tim23 contains two independent import signals. One is located within the first 62 amino acid residues of the hydrophilic domain that, in the assembled protein, is exposed to the intermembrane space. This signal mediates translocation of Tim23 across the outer m...

Health care processes are nowadays heavily dependent on Information Technology (IT). On the other side, assessing a higher quality of health care to patients has become increasingly important. Effective health care processes are thereby heavily dependent on a comprehensive IT-support. Therefore, a thorough understanding about both the hospital IT-systems and clinical pathways is required, to id...

RNA import into mammalian mitochondria is considered essential for replication, transcription, and translation of the mitochondrial genome but the pathway(s) and factors that control this import are poorly understood. Previously, we localized polynucleotide phosphorylase (PNPASE), a 3' --> 5' exoribonuclease and poly-A polymerase, in the mitochondrial intermembrane space, a location lacking res...

Ups1p, Ups2p, and Ups3p are three homologous proteins that control phospholipid metabolism in the mitochondrial intermembrane space (IMS). The Ups proteins are atypical IMS proteins in that they lack the two major IMS-targeting signals, bipartite presequences and cysteine motifs. Here, we show that Ups protein import is mediated by another IMS protein, Mdm35p. In vitro import assays show that i...

Protein import to the nucleus is a signal-mediated process that exhibits saturation kinetics. We investigated whether signal bearing proteins compete with U2 and U6 snRNPs during import. When injected into Xenopus oocytes, saturating concentrations of P(Lys)-BSA, a protein bearing multiple nuclear localization signals from SV40 large T-antigen, reduce the rate of [125I]P(Lys)-BSA and of [125I]n...

The assembly of eukaryotic ribosomal subunits takes place in the nucleolus and requires nuclear import of ribosomal proteins. We have studied this import in a mammalian system and found that the classical nuclear import pathway using the importin alpha/beta heterodimer apparently plays only a minor role. Instead, at least four importin beta-like transport receptors, namely importin beta itself,...

In 1965, Gus Dinizulu, an African American percussionist, traveled to Ghana with the dance company he was leading. There, took trip as opportunity explore his roots and met Nana Oparebea, Ghanaian chief-priestess of Akonedi Shrine, north Accra. She performed for Dinizulu a divination, during which she explained that enslaved ancestors were part akan people gave him mission search other American...

Sensor networks that can support time-critical operations pose challenging problems for tracking events of interest. We propose an architecture for a sensor network that autonomously adapts in real-time to data fusion requirements so as not to miss events of interest and provides accurate real-time mobile target tracking. In the proposed architecture, the sensed data is processed in an abstract...