The aim of this study was to investigate the effects of in ovo injections of albumin, amino acid and dextrose into the amnion on the hatchability, growth performance and slaughter yield of Ross 308 broiler chicks. Fertile eggs (n=360) were assigned to 6 treatment groups (4 replicates of 15 eggs each) including: non-injected eggs (control), and eggs injected with 0.7 ml distilled water (sham), a...

Betaine alleviates high-fat diet-induced fatty liver and prenatal betaine programs offspring hepatic lipid metabolism. Excessive corticosterone (CORT) exposure causes fatty liver in chickens, yet it remains unknown whether and how prenatal betaine modulates the susceptibility of CORT-induced fatty liver later in life. In this study, fertilized eggs were injected with saline or betaine before in...

The gypsy retrovirus of Drosophila is quite unique among retroviruses in that it shows a strong preference for integration into specific sites in the genome. In particular, gypsy integrates with a frequency of > 10% into the regulatory region of the ovo gene. We have used in vivo transgenic assays to dissect the role of Ovo proteins and the gypsy insulator during the process of gypsy site-speci...

background: in this feasibility study, a mechanically loaded in-vitro tissue culture model of joint morphogenesis using the isolated lower extremity of the 8 day old chick embryo was developed to assess the effects of mechanical loading on joint morphogenesis. methods: the developed in-vitro system allows controlled flexion and extension of the chick embryonic knee with a range of motion of 20 ...

the study of interactions of jet into cross flow at different longitudinal and transverse angles ofjet was studied. the following components were designed and constructed: a low velocity windtunnel to produce the uniform flow, a flat plate with a traverse injection system to simulate the jetinjection, and a spatial rake to measure the total pressure. the tests were carried out atlongitudinal (α...

Cytosine-guanosine deoxynucleotides (CpG) DNA can be delivered in ovo at embryo day (ED)18 for the stimulation of toll-like receptor (TLR)21 signaling pathway that ultimately protects chickens against a number of bacterial and viral infections. There is a dearth of information understanding the mechanisms of protection induced by in ovo delivered CpG DNA. The objective of this study was to dete...

AIM We analysed the effects of acute temperature change on the beating rate, conduction properties and calcium transients in the chick embryonic heart in vitro and in ovo. METHODS The effects of temperature change (34, 37 and 40 °C) on calcium dynamics in isolated ED4 chick hearts in vitro were investigated by high-speed calcium optical imaging. For comparison and validation of in vitro measu...

The characterization of human stem cells for the usability in regenerative medicine is particularly based on investigations regarding their differentiation potential in vivo. In this regard, the chicken embryo model represents an ideal model organism. However, the access to the chicken embryo is only achievable by windowing the eggshell resulting in limited visibility and accessibility in subse...