Light is important for the production of metabolites by plant cell culture. It is therefore necessary to evaluate the light conditions with respect to exposure time. Both enhancement and inhibition have been observed depending on exposure time and species. Callus culture of D. nervosa was established and effect of light and passage on cardiac glycoside production was studied. In vitro cultures ...

entomopathogenic fungi produce secondary metabolites which may bioactively help fungus in its virulence toward insect hosts. beauveria bassiana produces several toxic low molecular compounds in vitro as well as in vivo, the most important of them is beauvericin. the beh isolate of the fungus was selected for beauvericin assay. beauvericin was obtained from surface and submerged cultures of the ...

An alternative in vitro approach to drug screening has been the use of human cell cultures for antiviral agents and microbial cell cultures for the assessment of the carcinogenic potential of selected compounds. A number of protozoan species have been also used as drug screens for anti-protozoal agents. The ciliated protozoan Tetrahymena pyriformis species has been widely utilised as a dru...

linum persicum is an endangered plant native to iran, from linaceae family. phytochemical analysis has shown that l. persicum in vitro cultures contain podophyllotoxin (ptox), the pharmacological precursor for anticancer drugs such as etoposide, etopophos, and teniposide. shoot culture of l. persicum was established from callus cultures in ms medium containing ga3. a study was designed to inves...

Background: Plants are an important natural source of compounds used in cancer therapy. Pancratium maritimum contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of P. maritimum extracts on MDA-MB-231 human epithelial adenocarcinoma cell line and on normal lymphocytes in vitro. Methods: Leaves, flowers, roots, and bulbs of P. mar...

nanotechnology is a rapidly growing field having potential applications in many areas. nanoparticles (nps) have been studied for cell toxicity, immunotoxicity, and genotoxicity. tetrazolium-based assays such as mtt, mts, and wst-1 are used to determine cell viability. cell inflammatory response induced by nps is checked by measuring inflammatory biomarkers, such as il-8, il-6, and tumor necrosi...

candidiasis , the main opportunistic fungal infection has been increased over the past decades. this study aimed to characterize c.albicans species complex ( c.albicans , c.dubliniensis , and c.africana ) isolated from patients with respiratory infections by molecular tools and in vitro antifungal susceptibilities by using broth microdilution method according to clsi m27-a3 guidelines. totally,...

objective(s):cutaneous leishmaniasis is a common and endemic disease in khorasan province in north-east of iran. the pentavalant antimony (sb v) is the mainstay of treatment that has many side effects and resistance to the drug has been reported. the microbicidal effect of ozone was proven in different microorganisms. since there is no study in this respect and to achieve a low cost and effecti...