Energy balance is regulated by ghrelin that is a neuroendocrine modulator. Ghrelin is expressed in reproductive organs. However, the role of ghrelin during in vitro maturation and bovine preimplantational development is limited. The purpose of this study was to determine the expression of ghrelin (GHRL) and its receptor growth hormone secretagogue receptor 1A (GHS-R1A) mRNA expression, and cumu...

Complete functional oocyte maturation involves many complex processes that occur within preovulatory follicles and are initiated and sustained by the gonadotropins, steroid hormones and many recognized and unrecognized factors. The product of oocyte maturation is a mature egg that has complete nuclear, plasma membrane and cytoplasmic changes that enable normal fertilization and subsequent devel...

background: dendritic cells (dcs) are professional antigen presenting cells that have an important role in the initiation of immune response. the use of maturation factors in dendritic cell differentiation provides a promising approach in immunotherapy. objective: in this study, we compared tumor necrosis factor-α, polyribocytidylic acid, lipopolysacharide and cpg oligonucleotides in inducing d...

objective: cryopreservation of ovarian tissue or follicles has been proposed as an alternative method for fertility preservation. although successful vitrification of follicles has been reported in several mammalian species, the survival rate is generally low. the aim of this study was to investigate the effects of fibroblast growth factor (fgf) and epidermal growth factor (egf) on in vitro pre...

in vitro maturation (ivm) is emerging as a popular technology at the forefront of fertility treatment and preservation. however, standard in vitro culture conditions usually increase reactive oxygen species (ros), which have been implicated as one of the major causes for reduced embryonic developmental competence. it is well-known that higher than physiological levels of ros triggers granulosa ...

The study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gFF) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in Black Bengal goats. Follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. Media were prepared using TCM-199 supplemented wi...

background: therapeutic potential of in vitro maturation (ivm) in infertility is growing with great promise. although significant progress is obtained in recent years, existing ivm protocols are far from favorable results. the first aim of this study was to investigate whether two step ivm manner change reactive oxygen species (ros) and total antioxidant capacity (tac) levels. the second aim wa...

introduction: dehp [di(2-ethylhexyl) phthalate)] is widely used in plastic industry and some reproductive toxicity has been shown with it. so, this study was designed to evaluate dehp effects on resumption of meiosis and in vitro maturation of mouse oocytes as well as development of embryos resulted from them. material and methods: mice of 4-6 weeks old were administered daily doses of 50, 100,...

One of the most effective ways to propagate Lilium is by using tissue culture techniques, preferably through bulblet production. In addition to the surviving percentage of Lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing Lilium micropropagation. Different concentratio...