abstract: introduction & objective: the infertility treatment by using in vitro matured oocyte has many potential applications. it minimizes the risk of ovarian hyper stimulation and is an alternative treatment for women with polycystic ovarian syndrome who may have problems regarding stimulation for ivf. this method may prove important for subjects in need of fertility preservation and also pr...

Background: Polycystic ovary syndrome (PCOS) is characterized by chronic anovulation and hyperandro genism and affects approximately 5-10% of women of reproductive age. In vitro maturation (IVM) of mammalian oocytes has become an efficient method to produce mature oocytes in order to use in assisted reproductive techniques such as in vitro fertilization (IVF), intra cytoplasmic sperm injection ...

Efforts have intensified to successfully mature and inseminate oocytes in vitro and then culture ensuing embryos to transferable stages from a large number of mammalian species. Success varies, but generally even for the most successful species it is only possible to obtain a maximum of a 40 to 50% development of zygotes to the blastocyst stage. Reduced oocyte developmental competence is sugges...

Previous our studies have shown that CD44, the principal receptor for hyaluronan, is present on cumulus cells during oocyte maturation. Although hyaluronan-CD44 interaction has been implicated in cumulus expansion and/or oocyte maturation, the full significance of CD44 remains unknown. The objective of the present study was to further investigate the role of CD44 in cumulus expansion and oocyte...

the development of culture media able to mimic the preovulatory stage of follicular environment and support nuclear and cytoplasmic maturation of oocyte is important for in-vitro fertilization (ivf) programs. it seems that the best culture media for embryonic development and cell cleavage is the natural composition which surrounds the oocyte which has been used occasionally in human ivf program...

in vitro maturation (ivm) is emerging as a popular technology at the forefront of fertility treatment and preservation. however, standard in vitro culture conditions usually increase reactive oxygen species (ros), which have been implicated as one of the major causes for reduced embryonic developmental competence. it is well-known that higher than physiological levels of ros triggers granulosa ...

Cyclic nucleotides cAMP and cGMP are among the main molecules that control maturation of mammal oocytes. The in vitro simulated physiological oocyte (SPOM) method was used to model accumulation oocyte. In current study, we preincubated oocytes cows (not primed with FSH) modulators: N 6,2’-O-dibutyryladenosine 3’,5’-cyclomonophosphate (dbcAMP) 3-isobutyl-1-methylxanthine (IBMX). use SPOM increas...

Oocyte maturation depends on multiple variables. In fact, in dogs, the requirements for oocyte development in vitro are so complex that achievement of female gamete maturation has been viewed as a difficult problem. The conundrum, thus far unanswered, of achieving dog oocyte maturation in vitro, is nowadays the major puzzle of the in vitro program in the canine species. In in vitro studies of d...

the aim of this study was to investigate the effect of different concentration of royal jelly’s fatty acids (0.0, 125, 250, 500, 1000 µg/ml and 50 µl dmso) on in vitro maturation of goat oocyte. goat ovaries were collected from local slaughterhouse and transported to the laboratory and cocs were cultured into the maturation media supplemented with different concentration of royal jelly’s fatty ...