BACKGROUND Coxiella burnetii is the causative agent of Q fever, a worldwide zoonosis which shows a wide diversity of clinical manifestations. The objective of this seroepidemiological study was to estimate the prevalence of antibodies to C. burnetii in a representative population of the Eastern area of the Cantabrian Community (Northern Spain). METHODS A cross-sectional study was conducted in...

Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV in chronic nephritis, little is known about its biological characteristics. In this study, we established a quantitative assay of FmoPV by using an indirect immunofluorescence technique. Viral titers of FmoPV were determined in one week. Tre...

We examined 138 Escherichia coli strains isolated from cultures of blood specimens from urosepsis patients (n = 78) and nonurosepsis patients (n = 30) and from fecal specimens of a healthy control group (n = 30) for P fimbriation with a mannose-resistant hemagglutination test, an indirect immunofluorescence assay, and a new commercial particle agglutination test (PF TEST; Orion Diagnostica, Esp...

Two hundred and thirty children clinically diagnosed as suffering from acute respiratory infection were tested for four major groups of viral aetiological agents, i.e. influenza para-influenza, respiratory syncytial virus (RSV) and adenoviruses using indirect immunofluorescence technique. At least one of the respiratory viruses was identified in 51 (22 per cent) specimens, which included influe...

It is a particular advantage of the indirect fluorescent antibody (IFA) method that antibodies of different immunoglobulin classes may be separately detected and measured by the use of monospecific antiglobulin conjugates. Matuhasi, Mizuoka, and Usui (1966), Kiraly, Bachhausz, Jobbagy, Lajos, and Kovats (1968), Julian, Logan, and Norins (1969), and Atwood and Miller (1969) studied antibodies in...

Indirect immunofluorescence assay titers of human sera obtained against the Legionella pneumophila serogroup 1 antigen (Philadelphia 1 strain) killed with 10% Formalin showed a tendency to be lower than those obtained against the reference heat-killed antigen (geometric mean titer of 194 and 370, respectively) when all other variables in the test were held constant. Test results were interprete...

visceral leishmaniasis is the second most common cause of fever of unknown origin in our study. this disease is not common in adults, although it's endemic among the pediatric age group. the majority of the affected individuals were young. high grade spiking fever, chills and splenomegaly were unique findings. other common findings were neutropenia, anemia, abnormal liver function tests, s...

Toxoplasmosis is a disease caused by a ubiquitous,obilgate intrace;;ular protozoan"Toxoplasma gondii".the present study was undertaken to determine the prevalence of Toxoplasma infection in Kerman province of Iran.Atotal of 690 bllod samples,collected from patients in Kerman hospitals were serologically tested by indirect fluorescent antibody technique(IFA) for Toxoplasma antibadies.46.9 percen...

The quality of commercial toxoplasmosis indirect fluorescent-antibody reagents was evaluated over a 6-year period. Seven manufacturers voluntarily submitted their products for evaluation in the Center for Disease Control Premarket Evaluation Program. Each product was tested in accordance with the Center for Disease Control performance and labeling specifications and evaluation methods. Only 49%...