The sequence of histogenesis and organogenesis in the calus of Hyssopus officinalis nodal explants was studied during treatment with growth regulators. This process represents an important indicator of the accommodation capacity of regenerated plantlets to the ex vitro conditions. The cell multiplication rate was stimulated in Murashige and Skoog medium supplemented with indole-3-butyric acid (...

Multiple shoots were obtained from nodal segments of mature trees of Elaeagnus angustifolia L. cultured on MS medium (Murashige and Skoog 1962) supplemented with 0, 0.88 or 2.22 micro M N(6)-benzyladenine. When nodal segments taken from the in vitro proliferated shoots were cultured under the same conditions, additional multiple shoots were obtained. Rooting of the in vitro propagated shoots wa...

AIastradTechniques for vegetative propagation of 10-year-old blue SP.'Uce (Picea pungens EngeJm.) by stem cuttings were investigated. Month of collection of the cuttings and application of rooting hormones were examined. In addition, cutting position, cutting length and caliper were examined in relation to rooting response. December was the best collection date for rooting and root production. ...

The present study was conducted in Bangalore. It aims at studying the effect of different concentrations indole-3-butyric acid (IBA) (1000, 2000 and 3000 ppm) on V-1 mulberry cuttings with varying number buds (one, two, three four) consist twelve treatments which were replicated thrice Factorial CRD. results interaction various concentration IBA recorded best budded treated ppm regarding sprout...

Capillary electrophoresis (CE) coupled with mass spectrometry (MS) or tandem mass spectrometry (MS/MS) is reported as an alternative and potentially useful method for the simultaneous analysis of various classes of phytohormones with diversified structures, including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), abscisic acid (ABA), gibberellic acid (GA), zeatin (Z), N-benzyladenine ...

Sericostoma pauciflorum Stocks ex Wight(Family Boraginaceae) used against cancer, diabetes and known to be health promoter. Callus cultures have been established from the stem explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg/L concentration of different growth hormone viz. kinetin (Kn), indole 3-acetic acid (IAA) and indole 3-butyric acid (IBA). At 6 weeks of age, these call...

Adventitious root formation in stem cuttings is affected by exogenous and endogenous factors. The study assessed the effect of Fe(III)-EDDHA (ethylenediamine-N,N-bis 2-hydroxyphenyl acetic acid) on rooting 4 indol-3-butyric acid (IBA)-treated hardwood aromatic medicinal species Rosmarinus officinalis. Cuttings treated with 0, 1000, 2000 or 3000 mg L?1 IBA were placed pots filled sand:perlite mi...

Abstract Corydalis saxicola Bunting, a medicinal herb known for alkaloids, grows mainly in Guizhou province and northwest Guangxi of China. In this study, the first time plant, an efficient adventitious shoot proliferation regeneration system via organogenesis from leaf petiole explants was established. Cytokinins, including 6-benzyladenine (BA) thidiazuron (TDZ), induced callus then organogene...