The current study was carried out for comparative estimation of phytochemicals, anti-radical properties and half maximal inhibitory concentration (IC50) seven species family Moraceae using 2, 2-Diphenyl-1-Picrylhydrazyl assay. Methanolic (70%) extraction crude leaves extracts done via Double Maceration technique. Quantitative assay determining the radical scavenging activity DPPH as well...

To study the bioactive substances of H. scabrum, ethanolic and aqueous extracts leaves flowers were analyzed for their phytochemical contents antioxidant actings. The potiential was estimated using four methods, including DPPH radical, ABTS decolorization assay, reducing power total capacity. results exhibited that flower contain higher amounts flavonoids (4.675 mg. g-1) saponin (29.572 mg.g -1...

Methotrexate was determined by the homogeneous enzyme immunoassay (EMIT) with the Multistat and CentrifiChem centrifugal analyzers and by the enzyme inhibition assay with use of the Multistat centrifugal analyzer. With both methods, the standard curve extends from 0.2 to 2.0 mumol/L and there is no interference from bilirubin in concentrations up to 100 mg/L. Moderately lipemic samples do not i...

Abstract: Background: Hyperuricemia and inflammation are associated with the etiology pathogenesis of several metabolic diseases. Controlling elevated levels uric acid can be a potential therapeutic option for Aim: The current study examined anti-inflammatory anti-hyperuricemic properties Ficus benghalensis bark extract in vitro using murine macrophage RAW 246.7 cells. Materials Methods: Hydroa...

Acetylcholinesterase (AChE), hydrolyzes acetylcholine to choline and acetate, thereby terminating this neurotransmitter effect at cholinergic synapses. Therefore, AChE inhibition is used for counterbalance the deficit in Alzheimer’s disease (AD) patients. In present work, order find new plant acetylcholinesterase inhibitors, hydroalcoholic extracts from seventeen medicinal species were screened...

Background: Cell ability to recover from radiation damage is of great relevance in cancer treatment. It is often believed that the inhibition of cell ability to the liquid holding recovery (LHR) may be an indicator of the overall suppression of cell ability to recover from potentially lethal radiation damage. However, the literature contains no experimental evidence whether the LHR inhibition m...

Background: Cassia absus L. and Citrus medica plants are sources of different phytochemicals with pharmaceutical significance. They biochemically distinctive used in producing various herbal products exclusive bioactivity. Also, the extraction these phytonutrients medicinal characteristics has recently increased. Objectives: We determined to evaluate plant-derived compounds such as polyphenols ...

Background: Phototherapies based on sunlight, infrared, ultraviolet, visible, and laser-based treatments present advantages like high curative effects, small invasion, negligible adverse reactions in cancer treatment. We aimed to explore the potential therapeutic effects of blue light emitting diode (LED) human hepatoma cells decipher underlying cellular molecular mechanisms. Methods:...

background micrornas are small and non-coding rna molecules with approximately 22 nt in length that cause inhibition of translation or degradation of mrna. mir-155 is a kind of molecule with different functions, such as its role in proliferation, apoptosis, inflammation, differentiation, and immunity. one of its best known functions is apoptosis that affects on caspase-3 activity. the main aim ...

An inverse relationship between the concentration of ribose 5-phosphate and apparent ribulose bisphosphate carboxylase activity was observed. The Lilley-Walker assay spectrophotometric assay, in which the 3-phosphoglyceric acid-dependent oxidation of reduced pyridine nucleotide is measured, is shown to be highly sensitive to inhibition by heavy metals. Analysis of the purity of reagents showed ...