A phosphodeoxyribomutase has been demonstrated in cell-free extracts of Sarcina lutea. The enzyme could be heated to 55 ° for at least 2 h and still retain activity. It had a pH optimum around 7.2, and the most highly purified preparation showed a specific activity of approx. 60 ~moles of deoxyribose 1-phosphate converted to deoxyribose 5-phosphate/mg protein/h. The enzyme was constitutive in t...

Inorganic phosphate may influence the adsorption of glyphosate to soil surface sites. It has been postulated that glyphosate sorption is dominated by the phosphoric acid moiety, therefore, inorganic phosphate could compete with glyphosate for surface sorption sites. We examine sorption of glyphosate in low organic carbon systems where clay minerals dominate the available adsorption sites using ...

Thirteen patients with hypercalcaemia due to carcinoma received inorganic phosphate, orally or intravenously, as palliative treatment for their high serum calcium levels. The serum calcium promptly fell in all patients fully treated, and there was a striking clinical improvement in most patients. The blood urea was usually unchanged or became nearer to normal, while the serum phosphate altered ...

As phosphate derivatives form such important intermediates between the functioning of tissues and the metabolism which supports function, we decided to investigate the levels of certain phosphates in brain slices before and during metabolism in vitro. Investigation of this type appeared all the more necessary because there is now an extremely interesting group of observations on the levels of p...

Phosphate stimulates penicillinase formation in Bacillus cereus 569. The rate of penicillinase synthesis in the presence of 0.3 m phosphate, pH 7.0, is approximately 10-fold greater than that for uninduced cells, while the rate of synthesis in the presence of 0.3 m phosphate and 1 unit/ml of penicillin is approximately fourfold greater than in the presence of penicillin alone. When phosphate-in...

Vol.33, No.2, 1987 in June 1986, after recahibration of the glucose method, gave results equal to the survey mean: 640 and 1110 mg/L, respectively. The addition of two more calibration points solved our problem. However, we do not want to imply that five or more calibration points are required. The important question is not how many calibration points are used, but rather, are the concentration...

GINN, H. EARL, AND LINDA L. SHANBOUR. Phosphaturia in magnesium-dejcient rats. Am. J. Physiol. 2 12(6) : 1347-1350. rg67.-The effect of magnesium deficiency on urinary phosphate excretion was studied in rats rendered magnesium deficient by diet. Increased urinary phosphate excretion was observed. Tubular maximum reabsorption (Tm) of inorganic phosphate was lower in magnesium-deficient (0.9 =t 0...

It has been shown that glycerol can be oxidized beyond the dihydroxyacetone (DHA) stage by resting Acetobacter suboxydans cells (1). Through washing and centrifuging of the cells at room temperature, most of the oxidative activity was destroyed, which, however, could be restored by the addition of inorganic phosphate and diphosphopyridine nucleotide (DPN). 2,4-Dinitrophenol (DNP), a phosphate “...

The calcium transport protein of the sarcoplasmic reticulum accepts inorganic phosphate rapidly when phosphorylation is initiated either by the addition of phosphate or magnesium ions to the calcium free protein. Phosphorylation proceeds much more slowly when it is initiated by the addition of the calcium chelatro ethyleneglycol-bis (beta-aminoethyl ether)-N,N'-tetraacetic acid (EGTA) to the ph...