The binding of dengue type 2 (DEN-2) virus to mammalian (LLC-MK(2) and Vero) and mosquito (C6/36 and AP61) cell surfaces was investigated by a virus-binding assay using purified (3)H-labeled DEN-2 virus. The DEN-2 virus binding to all four cell types was specific and saturable, indicating the presence of a single class of receptors (ranging from 3.7 x 10(3) to 3.5 x 10(4) receptors/cell) with a...

introduction: cutaneous leishmaniasis is a parasitological disease that is spread in many tropical and subtropical areas in world. this disease transmitted by female sand flies. it is clear that knowledge on sand flies ecology has the main role to planning of control of cl. this study was carried out for determination of cl vectors, in ghanavat district in qom province during 2012. material and...

BACKGROUND The Baculovirus Expression Vector System (BEVS) is a very popular expression vector system in gene engineering. An effective host cell line cultivation protocol can facilitate the baculovirus preparation and following experiments. However, the counting of the number of host cells in the protocol is usually performed by manual observation with microscopy, which is time consuming and l...

the organization of gall on the ovary ofsesamum indicum l induced by the gall midge, asphondylia sesami felt, not only affects biochemical equilibrium of the host but also causes disturbed vegetative growth and reduced seed-setting. the toxicity created by the gall maker induces the re-orientation of vasculature cecidogenetic stimuli lead to excessive hypertrophy and hyperplasy forming the gall...

the aphid species, cinara pini (linnaeus, 1758) reported in our previous work as a new aphid on pinus trees for iran, was described using the classic method and through analysis of coi gene sequence. in the next step, we addressed the efficiency of the entomopathogenic fungus, lecanicillium longisporum (zimm.) zare and gams strain lrc 190, on the aphid. the fungus was administered to the second...

zoonotic cutaneous leishmaniasis (zcl) is an increasing and important public health problem in iran. the use of repellents is recommended as one of the important means of personal protection against vectors of zcl. this paper reports the repellency effect of the plant myrtle, myrtus communis (myrtaceae), essential oil for protection against 3-7-day-old unfed females of the sandfly, phlebotomus ...

BACKGROUND Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. METHODS The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...

The keratinase (kerA) gene from Bacillus licheniformis PWD-1 was expressed and purified in insect cells. First, the sequence encoding Ker-His-Flag was designed based on the amino acid sequence of the protein and peptide and codon optimization in order to ensure the high expression in insect cells. In the next step, the synthetic DNA was inserted into the pUC57 vector and then sub-cloned in the ...

The kinetic effect of silkworm hemolymph on host cell viability during a baculovirus-induced insect cell death process was investigated. Host cell viability after viral infection is important for replication of the baculovirus DNA containing a recombinant gene and expression of the cloned gene. The baculovirus-induced insect cell death process can be divided into a delay phase and a first-order...