For whole-genome analysis in a basal chordate (protochordate), we used F1 pseudo-testcross mapping strategy and amplified fragment length polymorphism (AFLP) markers to construct primary linkage maps of the ascidian tunicate Ciona intestinalis. Two genetic maps consisted of 14 linkage groups, in agreement with the haploid chromosome number, and contained 276 and 125 AFLP loci derived from cross...

A survey for resistance against net blotch disease (caused by Pyrenophora teres) was performed on some Egyptian barley landraces and some selected resistance and susceptible standard German barley genotypes. The results indicated that most of the Egyptian barley landraces are extremely resistant to the disease. Molecular analysis using RAPD and AFLP showed unique banding profiles for the differ...

Currently, there is an increasing focus on understanding the interactions between genetic features of invader and environmental factors that ensure success invasion. The objective our study was to evaluate diversity Lithuanian populations highly invasive small balsam (Impatiens parviflora) by amplified fragment length polymorphism (AFLP) markers relate molecular data biotope defined employing n...

Twelve episodes of acute fatty liver of pregnancy (AFLP) were diagnosed in 11 patients during the past 18 years in a general hospital in Santiago, Chile, with a prevalence of 1 per 15,900 deliveries. Acute fatty liver of pregnancy started between the 31st and 38th weeks of pregnancy, with malaise, vomiting, jaundice, and lethargy as the main clinical manifestations. Polydipsia (in nine episodes...

Senescence is a genetically regulated oxidative process that involves a general degradation of cellular structures and enzymes and the mobilization of the products of degradation to other parts of the plant. The cDNA-AFLP (cDNA-Amplified Fragment Length Polymorphism) analysis has been used under stringent PCR conditions afforded by ligation of adapters to restriction fragments, and the use of s...

The apple fruit moth (Argyresthia conjugella (A. conjugella)) in Norway was first identified as a pest in apple production in 1899. We here report the first genetic analysis of A. conjugella using molecular markers. Amplified fragment length polymorphism (AFLP) analysis was applied to 95 individuals from six different locations in the two most important apple-growing regions of Norway. Five AFL...

The devastating dry rot fungus, Serpula lacrymans, has a worldwide occurrence in buildings. We investigated the genetic variation in European isolates belonging to five vegetative compatibility groups (VCGs) by AFLP analysis. Our results indicate that S. lacrymans in Europe is genetically extremely homogenous; only five out of 308 scored AFLP fragments (1.6 %) were polymorphic. In contrast, S. ...

Panax japonicus is an important medicinal plant. The aim of this study was to develop species-specific molecular markers for P. japonicus. Amplified fragment length polymorphism (AFLP) was compared among P. japonicus, P. ginseng and P. quinquefolius. A clear species-specific AFLP marker for P. japonicus was generated. After isolation and sequencing of the AFLP fragment, a DNA sequence (293 bp) ...

American Tegumentary Leishmaniasis is caused by parasites of the genus Leishmania, and causes significant health problems throughout the Americas. In Panama, Leishmania parasites are endemic, causing thousands of new cases every year, mostly of the cutaneous form. In the last years, the burden of the disease has increased, coincident with increasing disturbances in its natural sylvatic environm...