A monoclonalantibody(66C7) was preparedthat specifically binds human salivary amylase (EC 3.2.1.1); it cross reacts with human pancreatic amylase by less than 1%. Two procedures are described for determination of isoamylases in human serum with this antibody: an enzyme immunoassay for determining amylase of salivary origin, and a routine method in which this amylase is immunoprecipitated and th...

BACKGROUND Low serum amylase levels may reflect impaired exocrine-endocrine relationship in the pancreas. However, few clinical studies have addressed this issue. Therefore, in this epidemiological study, we investigated whether low serum amylase was associated with the pathogenesis of impaired insulin action: metabolic syndrome (MetS) and diabetes. RESEARCH DESIGN AND METHODS Serum amylase, ...

The present review describes the structural features of alpha-amylase, beta-amylase and glucoamylase that are the best known amylolytic enzymes. Although they show similar function, i.e. catalysis of hydrolysis of alpha-glucosidic bonds in starch and related saccharides, they are quite different. alpha-Amylase is the alpha --> alpha retaining glycosidase (it uses the retaining mechanism), and b...

Bacillus stearothermophilus grew better on complex and semisynthetic medium than on synthetic medium supplemented with amino acids. Amylase production on the complex medium containing beef extract or corn steep liquor was higher than on semisynthetic medium containing peptone (0.4%). The synthetic medium, however, did not provide a good yield of extracellular amylase. Among the carbohydrates wh...

in this paper, covalent immobilization of beta amylase enzyme on the surface of modified magnetic nano particles (znfe2o4@sio2-nh2) is reported. for doing so, at first, the magnetic nanoparticles of znfe2o4 were synthesized by chemical co-precipitation method and then tetraethyl orthosilicate (teos) and 3-aminopropyltriethoxy silane (aptes) were used for modification of znfe2o4 nanoparticles wi...

The mean amylase level in 42 human bile samples was 154 IU/l and there was no significant difference in the amylase activity of 32 paired serum and bile samples. Estimation of the amylase thermolability of bile showed it to be similar to that of serum. This suggests that the amylase activity in bile may have filtered through the liver from the hepatic circulation rather than refluxed from the p...

The relative levels of pancreatic amylase mRNA closely parallel the 3-fold variation of pancreatic amylase protein levels observed in several mouse strains studied. In contrast pancreatic elastase mRNA levels were similar in these strains. Each of the strains contained the same number of amylase-like genes (about 8). The selective variation in the pancreatic amylase gene expression could be due...

The gene for beta-amylase was isolated from BacUlus polymyxa by molecular cloning in B. subtils. B. subtlis cells containing this gene express and secrete an amylase which resembles the B. polymyxa beta-amylase and barley beta-amylase in terms of the products it generates during carbohydrate hydrolysis. Starch hydrolysis with this beta-amylase produces maltose, not glucose, whereas maltotriose ...

In 1988, Hata et al. [1] reported a case of ectopic production of salivary-type amylase by an IgA-type multiple myeloma. Subsequently, seven cases of amylaseproducing multiple myeloma have been reported [2-8]. According to Delannoy et al. [7], amylase-producing myeloma should be added to the list of amylaseproducing tumors. In 1991, we reported a neuraminidase sensitive, so-called sialyl, saliv...

We describe a family of 32 subjects, including two healthy siblings without detectable pancreatic amylase in serum and five healthy relatives (one of them a sister) exhibiting subnormal pancreatic amylase activities. Comparisons of immunoreactive amylase concentrations with the corresponding amylase activities may justify the assumption of a "silent" Amy-2 allelic variant.