IGS is abundant in polymorphism, which widely used the analysis of intraspecific genetic diversity and phylogenetic relationships among geographical populations. In this study, 45S rDNA repeat unit Saccharina japonica was obtained for first time by BAC clone sequencing. The total length S. 8 995 bp, including 5 420 bp 18S-5.8S–25S rDNA, 3 575 (Intergenic Spacer), with GC content 51.4 %. compose...

The ITS region from a wide taxonomic range of nematodes, including secernentean and adenophorean taxa, and free-living, entomopathogenic, and plant-parasitic species, was evaluated as a taxonomic marker. Size of the amplified product aided in the initial determination of group membership, and also suggested groups that may require taxonomic reevaluation. Congeneric species often displayed ident...

We have detected a set of transcripts in Drosophila melanogaster cells which are homologous to repeating elements within the 'non-transcribed' spacer region of rDNA. The RNA molecules range from 240 to 1680 nucleotides, differing in length by an integral value of 240 nucleotides. We have sequenced several AluI fragments which characterise the main 240 nucleotide repeating element. We find that ...

A reintroduced white-tailed sea eagle (Haliaeetus albicilla) in moderate body condition was found dead and submitted for post-mortem examination. There were no signs of disease on gross pathological Histopathological examination however revealed the presence encysted protozoan parasites pectoral cardiac muscle sections. Polymerase chain reaction amplification extracted genomic DNA sequencing fo...

Ribosomal DNA (rDNA) from Xenopus laevis contains several heterogeneities in all three transcribed spacers, as revealed by analysis of cloned and uncloned amplified rDNA from oocytes and cloned chromosomal rDNA from erythrocytes. Heterogeneities include single base changes and length variants of one to several nucleotides. Sites of variation are widely but non-uniformly distributed, some occurr...

The second internal transcribed spacer region of the nuclear ribosomal gene cluster (ITS2). This consists three genes (18S rDNA, 5.8S rDNA and 28S rDNA) which have into RNA. Nevertheless, not translated protein. It is sensitive to detect S. scabiei DNA even after treatment. In addition, this can genetic variations species that are related taxonomically. study aimed determine compare sensitivity...

Paratylenchus shenzhenensis n. sp. was collected from the rhizosphere soil of Anthurium andraeanum in Shenzhen, Guangdong Province, China. The new species is characterized by having a female with a small body (249-302 μm), well developed stylet (17-21 μm), rounded head with four submedian lobes and lip-region with a slight depression at the oral area, small post-vulval uterine sac with a few ve...

The genetic diversity and phylogeny of Bridgeoporus nobilissimus have been analyzed. DNA was extracted from spores collected from individual fruiting bodies representing six geographically distinct populations in Oregon and Washington. Spore samples collected contained low levels of bacteria, yeast and a filamentous fungal species. Using taxon-specific PCR primers, it was possible to discrimina...

ABC Fax + 41 61 306 12 34 E-mail [email protected] www.karger.com © 2002 S. Karger AG, Basel 0301–0171/02/0981–0078$18.50/0 Accessible online at: www.karger.com/cgr Abstract. In higher eukaryotes, the 5S ribosomal DNA (5S rDNA) is organized in tandem arrays with repeat units composed of a coding region and a non-transcribed spacer sequence (NTS). These tandem arrays can be found on either one or...