Since the introduction of intracytoplasmic sperm injection (ICSI) in 1992, the new technology has raised a series of basic moral issues. In this article, I present a brief review of this moral debate. I argue that even if we solve the moral problems of the debate, there is a residue of value issues. These value issues are crucial for the understanding of the dynamics of the health policy and th...

In mice and humans, a normal offspring can be obtained by injecting a single spermatozoon into an oocyte, the process called intracytoplasmic sperm injection (ICSI). When three or more mouse spermatozoa with intact acrosomes were injected into individual mouse oocytes, an increasing proportion of oocytes became deformed and lysed. Oocytes did not deform and lyse when acrosome-less spermatozoa w...

BACKGROUND Recently, motile sperm organelle morphology examination (MSOME) criteria as a new real time tool for evaluation of spermatozoa in intracytoplasmic sperm injection (ICSI) cycles has been considered. OBJECTIVE The aim was to investigate the predictive value of MSOME in in vitro fertilization (IVF) in comparison to ICSI cycles and evaluation of the association between MSOME parameters...

Artificial oocyte activation to overcome failed fertilization after intracytoplasmic sperm injection (ICSI) in human oocytes typically employs Ca2+ ionophores to produce a single cytosolic Ca2+ increase. In contrast, recombinant phospholipase Czeta (PLCz) causes Ca2+ oscillations indistinguishable from thoseoccurring during fertilization, but remains untested for its efficacy in a scenario of I...

Artificial oocyte activation to overcome failed fertilization after intracytoplasmic sperm injection (ICSI) in human oocytes typically employs Ca(2+) ionophores to produce a single cytosolic Ca(2+) increase. In contrast, recombinant phospholipase Czeta (PLCζ) causes Ca(2+) oscillations indistinguishable from those occurring during fertilization, but remains untested for its efficacy in a scenar...

Notwithstanding the thousands of seemingly healthy children born after intracytoplasmic sperm injection (ICSI), it is not yet possible to conclude absolutely that the ICSI procedure might induce some altered development or that the ICSI protocol might not be improved even further. To address this in a clinically relevant system, the developmental potential of rhesus monkey embryos produced by I...

background: recently, motile sperm organelle morphology examination (msome) criteria as a new real time tool for evaluation of spermatozoa in intracytoplasmic sperm injection (icsi) cycles has been considered. objective: the aim was to investigate the predictive value of msome in in vitro fertilization (ivf) in comparison to icsi cycles and evaluation of the association between msome parameters...

As assisted reproductive technology (ART) methods become the mainstream of infertility treatment, it has become even more critical to reassess its safety. Following the results of a study published by the Robinson Institute in the New England Journal of Medicine, the risk of ART, especially intracytoplasmic sperm injection (ICSI), has never been so closely scrutinized. This paper traces the ori...

Background Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmen...