Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...

The gene encoding the brain-derived neurotrophic factor (BDNF) has been repeatedly associated with human obesity. As such, it could also contribute to the regulation of energy partitioning and the amount of secreted milk fat during lactation, which plays an important role in milk production in dairy cattle. Therefore, we performed an association study using estimated breeding values (EBVs) of b...

data related to holstein dairy cattle from 1987 to 2004 were obtained from animal breeding center of iran to estimate the genetic and phenotypic trends. variance components and genetic parameters of the first lactation records were estimated using restricted maximum likelihood method and aireml algorithm through matvec program. animal breeding values were predicted through multiple-trait animal...

efficiency is defined as a concept that determines the input output ratios in an economic system. data envelopment analysis method is a useful tool to estimate the efficiency of a unit. the purpose of this study was to estimate technical efficiency and to determine return to scale of holstein cattle farms in guilan province. therefore, the information including the number of labor and dairy cow...

This study was conducted to estimate the potential acid production value (PAPV) of major diets and to determine the relationship between dietary PAPV and dairy production traits. Estimation of PAPV of major cattle feeds was based on an in vitro technique, which determined the degree of Ca dissociation from CaCO3. Data on feeds and production traits were collected on 744 multiparous lactating Ho...

An unusual occurrence of actinobacillosis was diagnosed in 4 heifers aged 8-15 months and 2 cattle in a dairy herd with 190 Iranian Holstein breed. Anorexia, dysphagia, drooling of normal or foodtinged saliva and presence of warts-like lesions on the dorsal surface of tongue shaft were observed in a 15-month-old heifer without showing protrusion of tongue or presenting woody tongue and no invol...

This experiment was conducted to determine the association between several risk factors which may affect culling of the Iranian Holstein cows. The data consisted of 329,772 lactation records collected by the Animal Breeding Center of Iran (2003 to 2012).The logistic regression was applied to investigate the associations between the response variable, the risk of culling, and predictor variables...

Genetic correlations between first calving interval (CI) and linear type traits in South African Holstein and Jersey cattle were estimated to assess the possibility of using type information as selection criteria for CI. All linear type traits routinely evaluated under the National Genetic Evaluation Programme (18 for Jersey and 17 for Holstein) were considered. Data were obtained from the Nati...

26 herds with 71 586 individual records were used. We tested a hypothesis that milk efficiency was influenced by the elevation of the farm, housing system, breed, area of altitude, and by the cooling of dairy cows. There were 20 herds from lowlands and 6 herds from mountains, 20 herds from free-stall housing, 6 herds from tie-stall housing. 8 herds consisted of Slovakian Pied cattle, 4 herds of...