The fruit trichomes of Cucurbitaceae are widely desired in many Asian countries and have been a key determinant cucumber (Cucumis sativus L.) cultivar selection for commercial production breeding. However, our understanding the initiation development is still limited. Here, we found that TINY BRANCHED HAIR (TBH) gene preferentially expressed multicellular trichomes. Overexpression CsTBH tbh mut...

The ability to adapt, survive, and compete with weeds of transgenic plants is the necessary evaluation content release lines in target regions. We compared weediness agronomic traits maize G1F-8 G1F-19 carrying mcry1F gene their near-isogenic inbred line Zheng 58 wasteland cultivated field under natural conditions for two consecutive years. results showed that there was no significant differenc...

Diffusates from seedlings and root exudates from 6-week-old plants of a monoecious line of cucumber, Cucumis sativus L., contained considerably higher levels of gibberellin-(GA-) like substances than did those from plants of an isogenic gynoecious line. Most of the GA-like activity was found in a chromatogram region typical of GA(1) and GA(3); some activity, particularly in root exudates, appea...

Induced pluripotent stem cell (iPSC) derived cardiomyocytes are an important model system for cardiac electrical disorders. They offer the potential to detect disease-specific differences in lines as well between healthy individuals of differing genetic backgrounds. However, variability iPSC cardiomyocyte differentiations makes these studies prone type 1 errors and optimal experimental designs ...

Intending to contribute sustainable agriculture by “New Green Revolution,” we developed a large-grain/semidwarf isogenic line “Koshihikari sd1GW2” that incorporates both the large-grain gene GW2 and semidwarf sd1. homozygous B3F2 plant with genomic background of Koshihikari was backcrossed twice sd1.” sd1GW2 fixed in BC5F3 found be 12.6 cm shorter than Koshihikari. Whole-genome sequencing prove...

A set of isogenic strains was constructed from the meningococcal reference strain H44/76 (B:15:P1.7,16) which differed only in their outer membrane protein (OMP) compositions. First, three isogenic strains lacking the expression of either class 3 (PorB) or class 4 (RmpM) OMP or both were obtained. Second, three isogenic class 1 OMP loop-deficient strains of H44/76 lacking the predicted loop 1 o...

Campylobacter jejuni is the leading cause of human gastroenteritis worldwide with over 500 million cases annually. Chemotaxis and motility have been identified as important virulence factors associated with C. jejuni colonisation. Group A transducer-like proteins (Tlps) are responsible for sensing the external environment for bacterial movement to or away from a chemical gradient or stimulus. I...

Many tumors overexpress the NQO1 gene, which encodes DT-diaphorase (NADPH:quinone oxidoreductase; EC 1.6.99.2). This obligate two-electron reductase deactivates toxins and activates bioreductive anticancer drugs. We describe the establishment of an isogenic human tumor cell model for DT-diaphorase expression. An expression vector was used in which the human elongation factor 1alpha promoter pro...