Elucidation of the energetic principles of binding affinity and specificity is a central task in many branches of current sciences: biology, medicine, pharmacology, chemistry, material sciences, etc. In biomedical research, integral approaches combining structural information with in-solution biophysical data have proved to be a powerful way toward understanding the physical basis of vital cell...

a thermodynamic study of copper ions by jack bean urease (jbu) was carried out at two temperatures of 27 and 37?c in tris buffer (30 mm; ph=7.0) using an isothermal titration calorimetry. there is a set of twelve identical and non-interacting binding sites for copper ions. the intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 285 µm and ?15.2 kj/mol at 27?c and 3...

Isothermal titration calorimetry (ITC) may be used to determine the kinetic parameters of enzyme-catalyzed reactions when neither products nor reactants are spectrophotometrically visible and when the reaction products are unknown. We report here the use of the multiple injection method of ITC to characterize the catalytic properties of oxalate oxidase (OxOx) from Ceriporiopsis subvermispora (C...

11 Isothermal titration calorimetry (ITC) was utilised to investigate suitability of the technique to determine 12 the stoichiometry and thermodynamics of the interactions that occur between a commonly used chemotherapeutic 13 drug, namely doxorubicin, and a polymer bead-based drug delivery embolisation system (DC BeadTM). Six 14 temperatures were selected for drug-polymer titrations (293 to 31...

Abstract Contamination with Cd, Cu and Pb is a major environmental issue. Cations of those metals may adsorb on negatively charged surfaces polymeric silicic acid (pSi), altering their fate. Aiming to elucidate the underlying mechanisms extent adsorption, we conducted batch adsorption experiments at pH 4 6 concentrations that excluded precipitation solid phases. Zeta-potential measurements were...

The interaction of biologicalmacromolecules, whether protein-DNA, antibody-antigen, hormone-receptor, etc., illustrates the complexity and diversity of molecular recognition. The importance of such interactions in the immune response, signal transduction cascades, and gene expression cannot be overstated. It is of great interest to determine the nature of the forces that stabilize the interacti...

We have investigated the thermodynamic parameters and binding of a regulatory subunit of cAMP-dependent protein kinase (PKA) to its natural low-molecular-weight ligand, cAMP, and analogues thereof. For analysis of this model system, we compared side-by-side isothermal titration calorimetry (ITC) with surface plasmon resonance (SPR). Both ITC and SPR analyses revealed that binding of the protein...