Primary features of hydrogen and carbon isotope fractionation during toluene degradation were studied to evaluate if analysis of isotope signatures can be used as a tool to monitor biodegradation in contaminated aquifers. D/H hydrogen isotope fractionation during microbial degradation of toluene was measured by gas chromatography. Per-deuterated toluene-d(8) and nonlabeled toluene were supplied...

Stable isotope geochemistry is a branch of geology that investigates the age of natural materials, their origin and the processes they have undergone since formation [1]. Stable isotope analysis is also used in biogeochemical studies to monitor element cycling in ecosystems [2] and to identify geographical/regional differences for food provenance and archaeology. Of the elements of interest in ...

The mechanism of N-methyltryptophan oxidase, a flavin-dependent amine oxidase from Escherichia coli, was studied using a combination of kinetic isotope effects and theoretical calculations. The 15(kcat/Km) kinetic isotope effect for sarcosine oxidation is pH-dependent with a limiting value of 0.994-0.995 at high pH. Density functional theory calculations on model systems were used to interpret ...

Isotopic analysis can be performed on minerals, rocks, soil, tissue, gases, and liquids – just about anything. In view of the broad possibilities, we will only cover a few generalities here. In most instances, the element to be analyzed must be first isolated and purified before it can undergo isotopic analysis in a mass spectrometer. Purification is necessary to avoid isobaric interferences an...

The mass and isotope dependence of limiting temperatures for hot nuclei are investigated. The predicted mass dependence of limiting temperatures is in good agreement with data derived from the caloric curve data. The predicted isotope distribution of limiting temperatures appears to be a parabolic shape and its centroid is not located at the isotope on the β-stability line(T=0) but at neutron-r...

Fractionation of stable carbon isotopes upon degradation of trichlorobenzenes was studied under aerobic and anaerobic conditions. Mineralization of 1,2,4-trichlorobenzene by the aerobic strain Pseudomonas sp. P51 which uses a dioxygenase for the initial enzymatic reaction was not accompanied by a significant isotope fractionation. In contrast, reductive dehalogenation by the anaerobic strain De...

Thermochemical sulfate reduction experiments with simple amino acid and dilute concentrations of sulfate reveal significant degrees of mass-independent sulfur isotope fractionation. Enrichments of up to 13‰ for (33)S are attributed to a magnetic isotope effect (MIE) associated with the formation of thiol-disulfide, ion-radical pairs. Observed (36)S depletions in products are explained here by c...

The mass and isotope dependence of limiting temperatures for hot nuclei are investigated. The predicted mass dependence of limiting temperatures is in good agreement with data derived from the caloric curve data. The predicted isotope distribution of limiting temperatures appears to be a parabolic shape and its centroid is not located at the isotope on the β-stability line(T=0) but at neutron-r...

The isotope shifts of forbidden transitions in Beand B-like argon ions are calculated. It is shown that only using the relativistic recoil operator can provide a proper evaluation of the mass isotope shift, which strongly dominates over the field isotope shift for the ions under consideration. Comparing the isotope shifts calculated with the current experimental uncertainties indicates very goo...