Background and Aims: Azoospermia factor (AZF) region of the Y-chromosome has several genes which are responsible for normal spermatogenesis. Microdeletions of these genes are associated with azoospermia and oligospermia. These microdeletions are too small to be detected by karyotyping. They can be easily identified using polymerase chain reaction. The aim of this study is to determine the frequ...

Abstract The present research aimed to investigate Loka Pere banana (Musa sp.), an endemic species with distinct characteristics, but currently underrepresented in scientific literature. Our primary goal was isolate the Internal Transcribed Spacer (ITS2) region from leaf and corm samples of this unique variant. As a methodological approach, we utilized modified CTAB protocol extract DNA amplifi...

In the Abruzzi village of Cocullo (Italy), each year, on May 1st, local snake hunters (known as Serpari) display colubrids, captured in wild, to commemorate ancient ritual San Domenico. The ascomycete Ophidiomyces ophidiicola (Oo) is causative agent ophidiomycosis, an emerging disease with sublethal effects. Skin lesions, such dysecdysis, edematous, crusty or necrotic scales, swellings, nodules...

background: the objectives of our research were to search for leishmania species in rodents in fars province, south of iran, and to compare molecular with conventional methods for detecting these para-sites. methods: rodents were captured using live traps and screened for leishmania species using molecular and conventional methods, including the taking of smears from each ear. nested pcr was em...

The two internal transcribed spacers (ITS) of the nuclear ribosomal (r) DNA tandem repeat were examined in ophiostomatoid fungi belonging to the genera Grosmannia and Leptographium and closely-related taxa. Although the DNA sequence of the ITS region evolves rapidly, core features of the RNA secondary structure of the ITS1 and ITS2 segments are conserved. The results demonstrate that structural...

this study included 81 samples which belonged to 13 speices from myriophyllum genus. both nrdna its1 and its2 and cpdna matk, rbcl and trnh-psba loci were used for diagnose of invasive from native plant. the nrdna its1and its2 data proved highly variable and could differentiate between all but had low pcr amplification success,based on these result they would not recommend as a suitable barcode...

Dorsal-spined protostrongylid nematode larvae (Metastrongyloidea: Protostrongylidae) were recovered from the feces of the endangered pampas deer (Ozotocerus bezoarticus celer) in Campos del Tuyú Wildlife Reserve, Bahia Samborombón, Argentina. Partial DNA sequences from the large subunit ribosomal RNA (LSU rRNA) gene and from the second internal transcribed spacer region (ITS2) were amplified, c...

A species-specific polymerase chain reaction (PCR) assay using primers already designed, based on differences in the nucleotides of the second internal transcribed spacer (ITS2), was used to identify the species composition of the Anopheles fluviatilis complex in Iran. All the amplified DNA samples obtained from specimens collected from different areas using different collection methods yielded...